摘要
目的:研制乙肝免疫传感器,用于临床检验。方法:以金电极为基底,优化实验条件,通过纳米金静电竞争吸附硫堇分子和乙肝过氧化酶酶标抗体。采用循环伏安法和线性扫描伏安法考察了传感器的性质。结果:对乙肝抗原进行了定量分析,线性范围为1∶30～1∶200;线性相关系数R为0.994。结论:用纳米金方法固定乙肝抗体制备的免疫传感器能应用于临床检验。
Objective To form Hepatitis B electrochemical immunosensor for the clinic detection. Methods Using the gold electrode as the fundus, the thionine and the HBV horseradish peroxidase（HRP） antibody were electrostaticly bounded to the self-assemble monolayer competitively to form the Hepatitis B electrochemical immunosensor. Cyclic vohammetry and linear scan vohammetry were used to investigate the chemical characteristics of the immunosensor. Results The linearity of Hepatitis B in the range of 1：30-1：200 with the correlation coefficient of 0. 994 was obtained. Conclusion The Hepatitis B electrochemical immunosensor formed by this way can be used to the clinic detection.
出处
《医疗卫生装备》
CAS
2007年第6期3-4,共2页
Chinese Medical Equipment Journal
基金
全军医学科研"十五"计划重点基金项目(02Z014)资助。
关键词
自组装
纳米金
免疫传感器
self-assembled
Au colloid
immunosensor
