摘要
目的：了解尼古丁对子宫内膜异位症（内异症）患者腹腔巨噬细胞（MΦ）释放肿瘤坏死因子α（TNF-α）和白细胞介素-8（IL-8）的影响.方法：分离培养12例内异症患者腹腔MФ,将终浓度分别为1、10、100、200μmol/L的尼古丁和终浓度为1nmol/L的雌二醇单独或共同加入细胞培养板,半小时内加入脂多糖（LPS）刺激,观察6、12、24、48、72h,用双抗体夹心酶联免疫分析法检测腹腔MΦ培养上清液中TNF-α和IL-8的水平.结果：尼古丁在1～ 200μmol/L范围内,能抑制内异症患者腹腔MΦ培养上清液中TNF-α的分泌（P＜0.01）.尼古丁浓度在100～200μmol/L范围内,能抑制IL-8的水平（P＜0.01）.尼古丁还能显著降低雌激素存在时TNF-α和IL-8的水平（P＜0.01）.结论：尼古丁能抑制内异症患者腹腔MΦ炎性因子的分泌,可能部分解释吸烟妇女内异症发病率低的原因,也可能为研究内异症的药物治疗提供新的思路.
Objective：To investigate the effect of nicotine on tumor necrosis factor α （TNF-α） and interleukin-8 （IL-8） production of peritoneal macrophages from patients with endometriosis. Methods：Peritoneal macrophages were obtained from 12 cases with endometriosis. Nicotine was added to peritoneal macrophages culture media 30 minutes before LPS, reaching final concentrations of 1、10、100μmol/L,and 200μmol/L,respectively. And estrodiol was added at the concentration of 1nmol/L alone or combined with nicotine. TNF-α and IL-8 levels in macrophage supernatant were detected by sandwich enzyme linked immunoassay. Results： Nicotine at the concentration between 1μmol/L and 200μmol/L inhibits TNF-α production in cultured macrophage supernatant （P 〈 0.01 ） and at the concentration between 100μmol/L and 200μmol/L inhibits IL-8 production （ P 〈 0.01 ）. Moreover, in the presence of estrogen, nicotine can still decrease the levels of TNF-α and IL-8 significantly （P 〈0.01 ）. Conclusions： Nicotine may suppress endometriosis by inhibiting cytokine release of peritoneal macrophages, which may partly explain why women who smoke have lower risk of endometriosis, and may provide a new strategy for treatment of endometriosis.
出处
《现代妇产科进展》
CSCD
北大核心
2007年第5期345-347,350,共4页
Progress in Obstetrics and Gynecology
