摘要
目的建立测定血浆、尿样本中丹皮酚含量的反相高效液相色谱法。方法血浆样品经沉淀蛋白,尿样经稀释后,以乙腈-0.1%磷酸pH3.5（55∶45）为流动相,经DiamonsilC18柱分离,进行色谱分析。结果本方法丹皮酚的血浆浓度在20-1000μg·L^-1,尿中浓度在20-800μg·L^-1内线性关系良好,方法回收率在81.0%-85.0%之间,日内、日间RSD均小于11.0%。结论本方法简便、快速、灵敏度高,可用于丹皮酚的药动学研究。
OBJECTIVE To establish a method for the determination of paeonol concentration in plasma and in urine. METHODS The analysis was conducted on a Diamonsil - C18 （ 4. 6 mm × 150 mm ,5 μm） column with mobile phase consisting of acetonitrile -0. 1% phosphate acid （55：45 ,adjusted to pH 3.5 with triethylamine）. RESULTS Good linearity was obtained in the concentration range of 20 - 1 000 μg·L^-1 of paeonol in plasma and 20 - 800 μg·L^-1 in urine. The average recovery was 81.0% - 85.0% at three different concentrations. The RSDs of intra- and inter-day were less than 11%. CONCLUSION This method is rapid, sensitive, precise,and can be used in the determination of paeonol in plasma and in urine for the clinical pharmacokinetic study.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2007年第11期863-865,共3页
Chinese Pharmaceutical Journal
