摘要
目的:考察和比较枯芩与子芩之间及其各自不同部位和不同炮制方法的黄芩苷含量。方法:采用反相高效液相色谱法,C18柱,以甲醇:水:磷酸(47:53:0.2)为流动相,检测波长为280nm,分别测定不同部位和不同炮制方法的枯芩与子芩中黄芩苷含量。结果:子芩比枯芩的黄芩苷含量高;在药典规定炮制方法中蒸法比煮法的含量高;两种黄芩的皮质部均比木质部的含量高。结论:炮制黄芩应保留药材皮部并应采用蒸法,这样所得黄芩苷含量较高且方法简便、快捷,应改变传统使用中对枯芩与子芩的看法。
Objective：To compare the content of baicalin of xylem-rottened Scutellaria and xylem-norottened Scutellaria on the different part and in the different preparing method.Methods：The contents of baicalin were determined by RP-HPLC. The Chromatographic conditions included hypersil C18 column and mobile phase consisting of mixture of methanol： water： phosphoric acid（47： 53：0.2）, detected wavelength is 280nm.Results：The content of baicalin of xylem-norottened ScuteUaria was more higher than xylem-rottened Scutellaria and the Scutellaria steamed before it were cutted to slices was more higher than that boiled and the xylem of Scutellaria more than the cortex of that.Conclusion：The method of preparing Chinese medicine for Scutellaria baicalensis should use steaming and the cortex of Scutellaria should be retained.
出处
《中国药物应用与监测》
CAS
2007年第2期23-25,共3页
Chinese Journal of Drug Application and Monitoring
关键词
黄芩
黄芩苷
反相高效液相色谱法
含量测定
Scutellaria baicalensis Georgi
Baicalin
RP-HPLC
Determination of contents
