摘要
目的了解分离自重症监护病房(ICU)铜绿假单胞菌(PAE)中,Ⅰ类整合子及抗菌药物耐药相关基因存在状况。方法对ICU分离33株PAE,采用PCR方法检测Ⅰ类整合子遗传标记(intⅠ1和qacE△1-sul1基因)及22种抗菌药物耐药相关基因。结果33株PAE中,oprD基因均存在缺失突变,其他23种基因intⅠ1、qacE△1-sul1、blaIMP、blaVIM、blaTEM、blaSHV、blaOXA-10群、blaCARB、blaPER、blaVEB、blaGES、blaDHA、blaOXA-1群、blaOXA-2群、blaBEL-1、blaCTX-M-1群、aac(3)-Ⅱ、aac(6′)-Ⅰ、aac(6′)-Ⅱ、ant(3″)-Ⅰ、ant(2″)-Ⅰ、catB和cml1均阴性。结论临床分离的PAE中不存在Ⅰ类整合子、oprD基因缺失突变率很高,oprD基因缺失突变是其对亚胺培南耐药的主要原因。
OBJECTIVE To investigate the class Ⅰ integron and the drug-resistant genes in Pseudomonas aeruginosa （PAE） isolated from ICU. METHODS Thirty three strains of PAE were isolated from hospitalized patients in ICU, and the class Ⅰ integron genetic markers （including int Ⅰ1 and qacE△1-sull） and 22 kinds of the drugresistant genes were analyzed by PCR. RESULTS In 33 strains of PAE, the deficiency rate of oprD gene was 100.0%, and genes of int Ⅰ 1,intⅠ1、qacE△1-sul1、blaIMP、blaVIM、blaTEM、blaSHV、blaOXA-10、blaCARB、blaPER、blaVEB、blaGES、blaDHA、blaOXA-1、blaOXA-2群、blaBEL-1、blaCTX-M-1、aac（3）-Ⅱ、aac（6′）-Ⅰ、aac（6′）-Ⅱ、ant（3″）-Ⅰ、ant（2″）-Ⅰ、, catB and cmll were all negative. CONCLUSIONS The class 1 integron is not existed in PAE isolates, and the deficiency rate of oprD gene is very high. oprD Gene lack mutation is the main mechanism of PAE resistant to imipenem.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2007年第5期496-498,共3页
Chinese Journal of Nosocomiology
关键词
铜绿假单胞菌
整合子
整合酶
遗传标记
耐药基因
Pseudomonas aeruginosa
Integron
Integrase
Genetic marker
Drug-resistant genes
