摘要
目的:观察依达拉奉对深低温保存大鼠断肢再植后出现缺血再灌注损伤时的保护作用。方法:实验于2006-04/11在山东省立医院手足外科低温医学实验室完成。①选取健康成年Wistar大鼠36只,随机分为对照组、冷冻组和依达拉奉组3组,每组12只。②对照组只显露股动静脉而不结扎,其他2组大鼠截断右后肢,对断肢进行深低温冷冻保存处理(自结扎股动静脉至深低温保存约2h)。③1个月后,将冷冻保存的断肢复温、灌洗液洗脱,依达拉奉组所用灌洗液中含依达拉奉0.5mg/kg,行自体肢体回植(自液氮中取出肢体至恢复血供约2h),恢复肢体血供4h后取材,骨骼肌丙二醛含量,超氧化物歧化酶活性以及线粒体ATP酶活性,测定胫前肌含水量,光镜观察各组骨骼肌肌组织的结构改变。结果:36只全部进入结果分析。①骨骼肌丙二醛含量:依达拉奉组低于冷冻组[(10.37±1.25),(15.36±1.28)μmol/g,P<0.01]。②骨骼肌ATP酶和超氧化物歧化酶活性:依达拉奉组高于冷冻组[(206.2±45.2),(72.7±32.5)μkat/g;(83.9±5.2),(70.5±8.0)mkat/g;P均<0.01]。③胫前肌湿/干质量比值:依达拉奉组低于冷冻组(4.89±0.82,6.38±0.63,P<0.01)。④光镜结果显示依达拉奉组骨骼肌损伤程度明显轻于冷冻组。结论:依达拉奉可以降低肢体再灌注后肌肉组织中的氧自由基水平,减轻缺血再灌注对骨骼肌造成的损伤,对缺血再灌注骨骼肌具有保护作用。
AIM： To explore the protective effect of edaravone on the ischemia/reperfusion injury of replanted rat limb after cryopreservation.
METHODS： The experiment was accomplished at Cryogenic Medicine Laboratory at Hand and Foot Surgery Department of Shandong Provincial Hospital from April to November 2006.①Thirty-six healthy adult Wistar rats were selected and divided into 3 groups randomly： control group, cold storage group and edaravone group, with 12 in each group.②The ischemia/reperfusion injury of hind limb skeletal muscle in rat model was established in the cold storage group and edaravone group. The femoral artery and vein were separated and ligated individually, and the right hind leg was cut off promptly and then stored with profound hypothermia. Whereas in the control group, the femoral artery and vein was separated without ligation.③After one-month cold storage, the limb was rewarmed, flushed with perfusate （in the edaravone group, the perfusate contained 0.5 mg/kg edaravone）, and replanted. There were about 2 hours between the isolation of limbs from liquid nitrogen and the recovery of blood supply. After 4 hours reperfusion, the samples were collected to measure content of malondialdehyde （MDA） in skeletal muscle tissues of rats, and activities of superoxide dismutase （SOD） and mitochondrial ATPase, meanwhile, water percentage of tibial muscle were detected and morphology of skeletal muscle was observed under microscope.
RESULTS： Totally 36 healthy Wistar rats included in the experiment entered the final analysis.①Comparison on contents of MDA： Compared with the cold storage group, content of MDA in the edaravone group was decreased [（15.36±1.28）, （10.37±1.25） μmol/g, P 〈 0.01].②SOD and ATPase activities in edaravone group were higher than those in cold storage group [（206.2±45.2）, （72.7±32.5） μkat/g; （83.9±5.2）, （70.5±8.0） mkat/g; P 〈 0.01].③Tibial muscle wet/dry weight ratio was higher in edaravone group than in cold storage group （4.89±0.82, 6.38±0.63, P 〈 0.01）.④The result of microscope examination showed that morphological lesion in edaravone group was significantly slighter compared with that in cold storage group.
CONCLUSION： Edaravone can decrease the content of oxygen free radicals in the skeletal muscle after reperfusion, reduce the ischemia/reperfusion injury and protect the skeletal muscles after ischemia/reperfusion.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第8期1416-1418,I0001,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
