摘要
目的:观察环磷酰胺对小鼠骨生物力学的影响,观察补中益气汤和葡萄糖酸钙对环磷酰胺造成的小鼠骨质疏松的防治作用。方法:实验于2005-10/2006-01在广东医学院药理教研室完成。选取昆明种小鼠40只,体质量(20±2)g,雌雄各半,按体质量对等原则随机分为4组,正常对照组、环磷酰胺模型组、环磷酰胺合用补中益气汤组、环磷酰胺合用葡萄糖酸钙组,每组10只。补中益气汤的制备:称取炙黄芪200g,党参60g,炙甘草100g,白术60g,当归60g,升麻60g,柴胡60g,陈皮60g,切碎,加水煎煮2次,滤过,滤液浓缩至1700mL,冰箱保存备用。正常对照组灌胃给予生理盐水10mL/(kg·d),环磷酰胺模型组灌胃给予环磷酰胺20mg/(kg·d);环磷酰胺合用补中益气汤组灌胃给予环磷酰胺20mg/(kg·d)的同时灌胃给予补中益气汤20mL/(kg·d);环磷酰胺合用葡萄糖酸钙组灌胃给予环磷酰胺20mg/(kg·d)的同时灌胃给予葡萄糖酸钙0.5mg/(kg·d)。均自由饮水和进食标准饲料。于实验第30天称质量后,处死动物,取肝脏、脾脏、胸腺称质量,根据动物体质量计算器官指数,同时取右侧股骨供骨生物力学测定用。结果:40只小鼠均进入结果分析。与正常对照组比较,环磷酰胺模型组骨生物力学指标最大载荷、弹性载荷、刚度系数均减少[(12.11±2.06)N,(8.15±2.57)N,(45.89±6.33)N/mm;(14.98±1.54)N,(10.17±1.17)N,(56.34±9.49)N/mm,P<0.05,P<0.01]。与环磷酰胺模型组比较,环磷酰胺合用补中益气汤组、环磷酰胺合用葡萄糖酸钙组生物力学指标最大载荷、刚度系数增高[环磷酰胺模型组:(12.11±2.06)N,(45.89±6.33)N/mm;环磷酰胺合用补中益气汤组:(15.52±2.67)N,(57.68±11.19)N/mm;环磷酰胺合用葡萄糖酸钙组:(17.10±2.96)N,(63.69±11.11)N/mm,P<0.05,P<0.01]。与环磷酰胺合用葡萄糖酸钙组比较,环磷酰胺合用补中益气组表现为各生物力学指标与环磷酰胺合用葡萄糖酸钙组相似,差异无显著性意义。结论:环磷酰胺能使小鼠骨结构性质和材料性质下降,补中益气汤和葡萄糖酸钙均能对抗环磷酰胺导致的骨质量下降,预防骨质疏松。
AIM： To study effect of Cyclophosphamide on mechanical parameters in mice, and observe the preventive and curative effect of Buzhongyiqidecoction and glucobiogen on mice with osteoporosis induced by Cycrophosphamide.
METHODS： The experiment was conducted in the Department of Pharmacology of Guangdong Medical College between October 2005 and January 2006. Forty Kunming mice （half males and half females） with the body mass of （20±2）g ware randomly divided into four groups： Normal controlgroup; Cyclophosphamide model group; Cyclophosphamide combined with Buzhongyiqi group and Cyclophosphamide combined with glucobiogen group with 10 animals in each group. Preparation of Buzhongyiqi decoction： 200 g of Huangqi, 60 g of Dangshen, 100 g of Zhigancao, 60 g of Baishu, 60 g of Danggui, 60 g of Shengma, 60 g of Caihu and 60 g of Chenpi were chopped out and water-decocted twice, which was then filtered and concentrated to 1700 mL and stored in the refrigerator. Animals in the normal control group were intragastrically administrated with normal saline at 10 mU（kg·d）, and mice in the Cyclophosphamide model group were lavaged with Cyclophosphamide at 20 mg/ （kg·d）, and mice in the Cyclophosphamide combined with Buzhongyiqi group were lavaged with Cyclophosphamide at 20 mg/（kg·d） and Buzhongyiqidecoction at 20 mU（kg·d） simultaneously, while animals in the Cyclophosphamide combined with glucobiogen group were perused with Cyclophosphamide at 20 mg/（kg·d） and glucobiogen at 0.5 mg/（kg·d）. All animals were provided with normal stuff and drank freely. After 30 days, mice were weighed and executed to weigh the liver, spleen and thymus gland, and the ratio of organs was calculated according to the body mass of animals. Meanwhile, the right femoral bone was obtained for biomechanical determination.
RESULTS： Totally 40 mice were involved in the analysis of results. Compared with the normal control group, the maximum load, elastic load and rigidity coefficient of mice in the Cyclophosphamide model group were decreased [（12.11±2.06） N,（8.15±2.57） N,（45.89±6.33） N/mm;（14.98±1.54） N,（10.17±1.17） N,（56.34±9.49） N/mm,P 〈 0.05,P 〈 0.01]. Compared with Cyclophophamide model group, the maximum load and rigidity coefficient in Cyclophosphamide combined with Buzhongyiqi group and Cyclophosphamide combined with glucobiogen group ware increased [Cyclophosphamide model group： （12.11 ±2.06）, （45.89±6.33）;Cyclophosphamide combined with Buzhongyiqi group： （15.52±2.67）,（57.68±11.19）;Cyclophosphamide combined with glucobiogen group：（17.10±2.96）,（63.69±11.11）,P 〈 0.05, P 〈 0.01]. All biomechanical indexes in Cyclophosphamide combined with Buzhongyiqi decoction group were similar to those in Cyclophosphamide combined with glucobiogen group, and there was no significant difference.
CONCLUSION： Cyclophosphamide can reduce the bone constitutive properties in mice, while Buzhongyiqi and glucobiogen can prominently improve the biomechanics properties of bone in mice and prevent osteoporosis.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第6期1159-1161,1164,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
广东省科技计划项目(2005B10401037)~~
