摘要
目的探讨桂枝茯苓丸抑瘤作用及对T细胞亚群的影响。方法制备荷瘤小鼠模型,计算抑瘤率,FACS直接免疫荧光染色法测定T淋巴细胞表型CD4、CD8分子。结果中药组抑瘤率为38.93%。模型组、环磷酰胺组CD8分子表达降低,与正常组比较,差异有统计学意义(P<0.05)。中药组CD8分子表达升高,与模型组比较,差异有统计学意义(P<0.05)。模型组和环磷酰胺组CD4/CD8升高,与正常组比较差异有统计学意义(P<0.05),GFW可以降低荷瘤鼠CD4/CD8,与模型组相比,差异有统计学意义(P<0.05);但不能使之恢复正常,与正常组比较差异亦有统计学意义(P<0.05)。结论桂枝茯苓丸能促进CD8分子的表达,使荷瘤鼠异常分布的T细胞亚群趋于正常水平,提示桂枝茯苓丸可通过调节机体免疫功能发挥抑瘤作用。
Objective To detect the anti-tumor effect and the effect of GFW on the T cell sub-group in peripheral blood. Methods T^1 ： S180 mice sarcoma model was used to detect the inhibiting effects of GFW,Flow cytomertric was used tc determine the expression in T lymphocyte sub-group of CD4 and CD8. Results For S180 sarcoma, the inhibiting ratio of GFW was 38. 93%. The expression of CD8 decreased in the model group and CY group compared with the normal group （P 〈 0. 05）. CD8 showed increase in GFW group,there was difference between GFW group and model group （P 〈 0.05）. Conclusions GFW can regulate the level of T cell subgroup to normal by rising the expression of CD8. The anti-tumor function of GFW may be closely related with immunoregulation.
出处
《齐齐哈尔医学院学报》
2007年第5期519-520,共2页
Journal of Qiqihar Medical University
