摘要
目的:探讨泛素特异性蛋白酶(USP)2、14和泛素因子E4A(UBE4A)在卵巢浆液性囊腺癌及癌旁正常卵巢组织间的表达差异。方法:采用RFDD-PCR、半定量RT-PCR和免疫组织化学染色方法检测USP2、USP14和UBE4A在40例卵巢浆液性囊腺癌患者卵巢癌组织及其癌旁正常卵巢组织上的表达差异。结果:泛素特异性蛋白酶USP2、USP14和UBE4A在卵巢浆液性囊腺癌组织中高表达,而在正常卵巢组织中无表达或低表达。结论:USP2、USP14和UBE4A在卵巢癌组织中表达上调,提示卵巢癌中泛素-蛋白酶体系统活动明显增强。
AIM:The differential expression of USP2,USP14 and UBE4A between ovarian serous cystadenocarcinoma and adjacent normal tissues was investigated.METHODS:Restriction fragment differential display polymerase chain reaction(RFDD-PCR),semi-quantitative RTPCR and immunohistochemical staining were applied to analyze the differentially expressed genes and proteins of ubiquitin specific proteases(USPs),USP2 and USP14,and ubiquitin factor E4A(UBE4A)between ovarian serous cystadenocarcinoma and adjacent normal tissues obtained from 40 patients aged from 29 to 72 years old,collected in 2005 year at excision of surgical operation with ovarian serous cystadenocarcinoma.RESULTS:USP2,USP14 and UBE4A were over-expressed(〉3 folds)in ovarian serous cystadenocarcinoma tissues compared to normal tissues,CONCLUSION:The results suggest that the activity of ubiquitin-proteasome system is obviously enhanced in ovarian cancer.
作者
杨瑛
侯建青
曲路云
王桂青
鞠红卫
赵志伟
于振海
羊惠君
YANG Ying;HOU Jian-qing;QU Lu-yun;WANG Gui-qing;JU Hong-wei;ZHAO Zhi-wei;YU Zhen-hai;YANG Hui-jun(Department of Anatomy,West China School of Preclinical and Forensie Medicine,Sichuan University,Chengdu 610041,China)
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2007年第6期504-506,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
烟台市科学技术发展计划项目基金资助(2006131-7)
关键词
卵巢癌
差异表达
RFDD-PCR
泛素-蛋白酶体系统
ovarian serous cystadenocarcinoma
differential expression
RFDD-PCR
ubiquitin-protea-some system
