摘要
选用特早熟杏红荷包为试材，首次将果实发育期45天的胚培养成株，从发育55～59天的胚得到300余株壮苗，栽植于选种圃．提高特早熟杏胚培成首的技术关键主要是：①选择适宜的接种时期，在花后59天硬核期PF1值达0.9时采样接种；②选用改良Tuber培养基，附加BAO．2毫克／升；③经80天以上0～3℃低温处理，④在15℃条件下，增强光照炼苗，9月下旬气温较低时移栽．
More than 300 vigorous plantlets have been got by culture of 55-59 d embryos from the ear-liest apricot variety Honghebao and transplanted in a breeding nursery. For the first time someplantlets have been cultured from the embryos at 45 d. The key techniques to increase percentageof plantlet formation were (i) sampling at 59 d after blossoming when the embryo was at stone-hardening stage with PF, being 0. 9, (ii) using the improved Turkey medium supplemented withBA (0. 2mg L-1), (iii) culturing at O-3℃ for more than 80 d, (iv) training at 15℃ with gradual-ly increased illumination, then transplanting to field in late September.
出处
《落叶果树》
1997年第1期3-6,共4页
Deciduous Fruits
关键词
特早熟
杏
品种
胚培养
earliest apricot, embryo culture
