摘要
以中华芦荟幼苗茎段为外植体,以MS为基本培养基,通过添加不同浓度的生长素、细胞分裂素或两者组合,以及添加Vc或活性炭(共350种组合),研究中华芦荟愈伤组织的诱导及生长情况。结果表明,在单独使用不同种类的生长素时,不同浓度的生长素诱导的愈伤组织频率差异明显,且愈伤组织的生长状态也各异;单独使用细胞分裂素时,愈伤组织的诱导率极低。当组合不同浓度和种类的生长素和细胞分裂素时,愈伤组织诱导频率差异显著;同时发现在减轻愈伤组织褐化的效果上,Vc较活性炭好。通过对愈伤组织诱导结果的比较,MS+2.0 mg/L 6-BA+0.5 mg/L 2,4-D+10 mg/L Vc为中华芦荟愈伤组织诱导的较好培养基,愈伤组织诱导率达到91.4%,且愈伤组织生长较旺盛。
Studies were given to the induction and growth young stem was applied as explant. MS was taken as IBA, NAA and 2, 4 - D）, cytokinins （6 - BA, KT of callus of Aloe vera L. var. chinensis （ Haw. ） Berg when the basic medium, added with various concentrations of auxins （ IAA, and Zeatin） and the different combinations of these plant growth regulators. Results showed that the callus is barely induced by cytokinin alone, and the callus induction rates have significant difference when different concentrations of auxin and different combinations of auxin with cytokinin are used on the MS medium. In order to irihibit callus browning during culturing, various concentrations of vitamin C or active carbon are added into four combinations of auxin and cytokinin respectively, which can induce callus most effectively. The result showsd that vitamin C can alleviate callus browning more effectively than the active carbon. The most efficient medium for induction and growth of callus of Aloe vera L. var. chinensis （ Haw. ） Berg was MS ＋ 2.0 mg/L 6 - BA ＋ 0.5 mg/L 2, 4 - D ＋ 10 mg/L vitamin C, with which the callus induction rate can reach to 91.4% and callus grow.vigorously.
出处
《重庆大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2007年第5期113-118,共6页
Journal of Chongqing University
基金
重庆市自然科学基金资助项目(8045
2004-56号)
关键词
中华芦荟
愈伤组织
诱导
生长素
细胞分裂素
Aloe vera L. var. chinensis （Haw.） Berg
callus
induction
auxin
cytokinin
