摘要
目的观察胆碱对离体大鼠心肌缺血/再灌注损伤的影响。方法采用Langendorff灌注系统,建立离体大鼠全心缺血/再灌注损伤模型。观察胆碱对复灌后冠脉流量、心肌组织丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性和心肌梗死面积的影响。结果胆碱可明显增加缺血/再灌注后1、5、10min的冠脉流量,减少心肌组织MDA含量、提高SOD活性,缩小心肌梗死面积,与模型组比较差异均有显著性(P<0.05)。上述作用可部分的被选择性M3受体阻断剂4-di-phenylacetoxy-N-methylpiperidine-methiodide(4-DAMP)所逆转。结论胆碱对离体大鼠心肌缺血/再灌注损伤具有保护作用。
Aim To study the influence of choline on ischemia-reperfusion injury （IRI） in isolated rat heart. Methods The isolated rat heart models of IRI were developed by linking the isolated rat heart to Langendorff perfusion system and 25 min of ischemia followed by reperfusion for 30 min. The outflow of coronary artery, the Maleic Dialdehyde （MDA） content and Superoxide Dismutase （SOD） activity were measured. Results In the presence of choline, the outflow of coronary artery （P 〈 0. 05 or P 〈 0. 01 ） and the SOD activity （P 〈 0. 01 ） were increased, and the MDA content was decreased after reperfusion （ P 〈 0. 05 ） , and the infarct size were also found to depress. The protective effects of choline on IRI could be reversed by 4-diphenylacetoxy-N-methylpiperidine-methiodide （4-DAMP）, a selective antagonist of M3 receptor. Conclusion A protective effect of choline on myocardial ischemia-reperfusion injury in rats in vitro was observed.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2007年第5期600-603,共4页
Chinese Pharmacological Bulletin
基金
国家重点基础研究发展规划(973规划)资助项目(No2004CCAO6700)
2003年国家自然科学基金面上项目(No30371647)
黑龙江省卫生厅资助项目(No2004-189)
黑龙江省青年科学技术专项资金资助项目(NoQC05080)
关键词
胆碱
心肌
缺血/再灌注损伤
4-DAMP
M3受体
choline
sion injury
4-DAMP
on injury in rats in vitro was obmyocardium
ischemia-reperfu M3 receptor
