摘要
目的探讨生长激素(growth hormone,GH)预处理对体外循环(cardiopulmonary bypass,CPB)大鼠肝脏炎性反应的影响及机制。方法建立大鼠CPB模型,分为GH预处理组(GH组)、CPB对照组(CPB组)和假手术组(SM组),按照CPB后不同时间取静脉血及肝组织,检测血清LPS、TNF-α浓度及肝功能,RT-PCR法检测肝组织CD14mRNA、TNF-α mRNA。结果CPB后CPB组、GH组LPS、TNF-α浓度比CPB前血清LPS(0.25±0.03)IU/L和TNF-α(0.95±0·38)μg/L显著升高,肝组织CD14mRNA、TNF-α mRNA的表达明显增多,肝功能损害明显;GH组同时相点血清LPS和TNF-α浓度以及肝组织CD14mRNA、TNF-α mRNA的表达均比CPB组显著降低(P<0·05,P<0·01),肝功能损害明显减轻。结论GH预处理可明显降低CPB后血清LPS水平,减少肝组织CD14mRNA、TNF-α mRNA的表达,从而降低血清TNF-α浓度,改善CPB后机体炎症反应,减轻肝损害。
Objective To study the effects and mechanisms of growth hormone （GH） pretreatment on the inflammatory response of liver in rats after cardiopulmonary bypass （CPB）. Methods The CPB model in rat was established. The rats were randomly divided into GH pretreatment group （GH group）, CPB group, and sham group. The serum levels of endotoxin, TNF-α, and liver function were determined at different intervals respectively. Simultaneously, the liver tissues were collected for the detection of the CD14 mRNA and TNF-α mRNA by RT-PCR. Results Compared with pre-CPB, the serum levels of endotoxin, TNF-α were significantly increased, and the lesion of liver function was apparent in GH group and CPB group. Meanwhile, the expression of CD14 mRNA and TNF-α mRNA in liver tissues were also increased obviously in both groups. However, the serum levels of endotoxin and TNF-α and the expression of CD14 mRNA and TNF-α mRNA in liver tissues at the same time point in GH group were significantly lower than those in CPB group （P 〈 0. 05 or P 〈 0.01 ）. Compared with CPB group, the liver function at the same time point improved in GH group. Conclusion GH pretreatment could decrease the serum levels of endotoxin and TNF-α, reduce the expression of CD14 mRNA and TNF-α mRNA in liver tissues, relieve inflammatory response and improve the liver function.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2007年第12期1224-1226,共3页
Journal of Third Military Medical University
关键词
生长激素
体外循环
内毒素
CD14
TNF-α
炎性反应
肝损害
growth hormone
cardiopulmonary bypass
endotoxin
CD14 mRNA
TNF-α mRNA
inflammatory response
liver injury
