摘要
目的:探讨HLA-DPB1等位基因与哮喘之间是否存在相关性,从基因水平探讨支气管哮喘的发病机制。方法:用聚合酶链反应-限制性片段长度多态性(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)分析法,检测四川汉族人HLA-DPB1基因多态性。用PCR技术对HLA-DPB1基因第2外显子(exon 2)进行体外扩增;然后用Ban II、Fok I、Dde I、Rsa I、EcoN I、BstU I 6种限制性内切酶对扩增产物进行酶切,电泳分离酶切片段,根据片段格局确定相应基因型别。结果:PCR扩增在327 bp有HLA-DPB1 exon 2目的基因产生。限制性内切酶酶切HLA-DPB1后,共检测到12种等位基因。HLA-DPB1*0201在哮喘患者组的出现频率显著低于对照组(P<0．05,RR=0．115<1)。结论:HLA-DPB1* 0201可能为支气管哮喘的抗性基因。
Objective：In order to study certain HLA-DPB1 alleles association with asthma and mechanism of asthma in gene level. Methods： HLA-DPB1 exon 2 gene was cloned in PCR, PCR products were electrophoresed in 2% agarose gel to validate the motive gene. Then HLA-DPB1 exon 2 gene was each cleaved by six restriction endonucleases including Ban Ⅱ, Fok Ⅰ, Dde Ⅰ, Rsa Ⅰ, EcoN Ⅰ, BstU Ⅰ and was directly electrophoresed according to RFLP. The HLA-DPB1 alleles were acquired, and the frequencies of them were compared between patients and normal controls. Results： The second DPB1 exon 2 and HGH gene were successfully amplified by PCR. The amplified DNAs were digested with restriction endonucleases. Here we got twelve DPB1 alleles . There was significantly decreased gene frequency of HLA-DPB1 ＊ 0201 in asthmatic patients compared with normal controls （P〈0.05, RR = 0. 115〈1）. Conclusion： HLA-DPB1 ＊ 0201 could be acting as IS gene and confer resistense to asthma.
出处
《中国临床医学》
北大核心
2007年第2期162-164,共3页
Chinese Journal of Clinical Medicine
基金
四川省教育厅资助项目(2003A057)
关键词
HLA—DPB1等位基因
哮喘
四川汉族
PCR—RFLP
HLA-DPB1 loci
Asthma
Sichuan Han pepole
Polymerase chain reaction-restriction fragment length polymorphism
