摘要
目的:研究金雀异黄素(Genistein)对人胃腺癌细胞SGC-7901中Akt及其磷酸化蛋白p-Akt (Ser473)和p-Akt(Thr308)表达的影响．方法:用浓度为0,5,10,20,40和80μmol／L的Genistein处理SGC-7901细胞24 h后,采用四甲基偶氮唑盐(MTT)方法检测Genistein对SGC-7901增殖的抑制作用,Hoechst 33342染色观察Genistein对SGC-7901凋亡的诱导作用,Western blot方法检测不同浓度Genistein处理后的SGC-7901细胞中Akt,p-Akt(Ser473)和p-Akt(Thr308)的表达情况．结果:5,10,20,40和80μmol／L的Genistein对SGC-7901细胞增殖的抑制率分别为6．85％±3．71％,13．19％±1．90％,20．94％±1．83％, 29．58％±1．19％和41．75％±1．92％．20-80μmol／L的Genistein处理组细胞呈现明显的凋亡形态学改变．Genistein对细胞中Akt蛋白的表达没有明显影响．不同浓度Genistein处理SGC-7901细胞24 h后,均有两种磷酸化Akt蛋白的表达,两种磷酸化蛋白表达均随Genistein浓度的增加而逐渐减弱,80μmol／L Genistein处理组表达最弱．结论:Genistein的抗癌活性与其抑制磷酸化Akt蛋白表达有关．
AIM： To investigate the effects of Genestein on the expression of Akt and its phosphorylated proteins （p-Akts） in human gastric carcinoma cell line SGC-7901.
METHODS： SGC-7901 cells were exposed to 0, 5, 10, 20, 40 and 80 μtmol/L Genistein for 24 hours. The effect of Genistein on cell proliferation was measured by methyl thiazolyl tetrazolium （MT1o assay, and the apoptosis of SGC-7901 cells was evaluated by Hoechst 33342 staining. The expression of Akt and p-Akts （Ser473 and Thr308） were measured by Western blot.
RESULTS： The inhibitory rate was 6.85% ± 3,71%, 13.19% ± 1.90%, 20,94% ± 1.83%, 29.58%± 1.19% and 41.75% ± 1.92%, respectively, as SGC-7901 cells were exposed to 5, 10, 20, 40 and 80 μmol/L Genistein for 24 hours. Typical morphological changes of apoptosis were induced by Genistein at the concentrations （20-80 μmol/L）. Genistein had no marked influences on Akt expression. Both Ser473 and Thr308 were expressed in SGC-7901 cells 24 hours after Genistein treatment, and they were decreased with the increasing of Genistein concentrations. The weakest expression appeared as 80 μmol/L Genistein was used.
CONCLUSION： The anti-cancer action of Genistein may be associated with the decreased expression of p-Akt （Ser473） and p-Akt （Thr308）.
出处
《世界华人消化杂志》
CAS
北大核心
2007年第10期1055-1059,共5页
World Chinese Journal of Digestology
基金
天津农学院科学研究发展基金资助项目
No.20030002~~
