摘要
目的 观察As2 O3 对胃腺癌细胞株SGC 790 1凋亡的诱导作用及凋亡细胞骨架的改变。方法 选用不同剂量As2 O3处理SGC 790 1细胞 ,分不同时间组 ,应用MTT法、光镜、透射电镜、TUNEL法和FCM法检测As2 O3 对细胞生长影响及对凋亡的诱导作用 ,应用荧光染色在激光共聚焦显微镜下观察凋亡细胞F actin和α tubulin的结构。结果 As2 O3 抑制该细胞株的生长 ,MTT法显示抑制程度具有时间和剂量效应关系。光镜及透射电镜观察发现 2 μmol/LAs2 O3 作用 96h后细胞数明显减少 ,并出现典型的凋亡形态。TUNEL法显示 2 μmol/LAs2 O3 作用后 ,凋亡细胞阳性率随时间延长而增加 (P <0 0 5 ) ,作用96h的阳性率为 (7 33± 1 83) %。FCA法显示 2 μmol/LAs2 O3 作用 96h后细胞的凋亡率为 8 4 8% ,并出现凋亡峰。激光共聚焦显微镜观察显示在细胞凋亡过程中 ,F actin和α tubulin均发生解聚和重排。结论 As2 O3 对胃癌细胞株SGC 790 1具有抑制生长及诱导凋亡的作用。
Purpose To investigate the effects of As 2O 3 on the cell growth of the SGC 7901 cell line and the redistribution of cytoskeleton of the apoptotic cells. Methods SGC 7901 cells were treated by different concentrations of As 2O 3 and with different duration. Cell growth and apoptosis were examined by MTT, light microscopy, transmission electron microscopy, TUNEL and flow cytometry. The changes of cytoskeleton were determined by the distribution of F actin and α tubulin in apoptotic cells by laser confocal scan microscope(LSM). Results MTT showed the inhibiton of SGC 7901 cell growth induced by As 2O 3 was time and dose dependent. Cell growth inhibition and morphological changes were obvious in 2 μmol/L As 2O 3 at 96h and characteristic apoptosis could be observed by means of light and transmission electron microscopes. TUNEL showed the positive rate was significantly increased with time ( P< 0 05) in 2 μmol/L As 2O 3 group. The positive rate was(7 33±1 83)% at 96 h. At 96 h, the rate of apoptosis was 8 48% in 2 μmol/L As 2O 3 group and the apoptotic peak was showed by FCA detection. LSM showed that the depolymerization and rearrangement of F actin and α tubulin were coincided with changes of apoptotic cells. Conclusions As 2O 3 could significantly inhibit the SGC 7901 cells growth and induce apoptosis. The changes of microfilaments and microtubules in cells might play a positive role in the morphological changes of apoptosis.
出处
《临床与实验病理学杂志》
CAS
CSCD
2003年第4期416-419,共4页
Chinese Journal of Clinical and Experimental Pathology
