摘要
参照GenBmk已发表的山羊痘病毒(GPV)和羊口疮病毒(ORF)的核苷酸序列设计了两对引物,建立了一种可以快速鉴别山羊痘病毒和羊口疮病毒二重PCR方法,检测结果显示,对山羊痘病毒和羊口疮病毒可以分别扩增出413 bp和595 bp的特异性片段,经敏感性测定,最低能检测到4 pg的DNA。对广西的送检的山羊病料20份进行检测,其中7份可扩增出413 bp的山羊痘特异片段、1份可扩增出595 bp的羊口疮特异片段。该方法能在4 h内完成整个检测过程,不仅快速,而且特异强、敏感性高,很适合于快速诊断。
A multiplex PCR method was developed using two specific oligonucleotide primers designed according to the published gene sequences of goat pox virus and sore mouth virus, A specific 413 bp gene fragment of goat pox virus and a 595 bp fragment of sore mouth virus were amplified. This PCR method is rapid, specific and sensitive and could detect as low as 4 pg of viral DNAs from either goat pox virus or sore mouth virus.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2007年第5期394-396,共3页
Chinese Journal of Preventive Veterinary Medicine
关键词
山羊痘
羊口疮
二重PCR
鉴别
goat pox virus
sore mouth virus
multiplex PCR
differentiation
