摘要
以结缕草品种‘Zenith’(Zoysia japonica‘Zenith’)的种子为外植体,在附加1.0 mg.L-12,4-D和0.1mg.L-16-BA或2.0 mg.L-12,4-D的MS培养基上培养,愈伤组织诱导率较高,分别达到100%和92%。在添加了2.0 mg.L-12,4-D或1.0 mg.L-12,4-D和0.1 mg.L-16-BA的MS培养基上继代培养可诱导出胚性愈伤组织,诱导率分别为3.26%和14.52%。胚性愈伤组织在含0.01mg.L-12,4-D的1/2 MS分化培养基上的分化率和生根率都达到100%。
Mature seeds of Zoysia japonica ＇Zenith＇ were used as initial explants for callus induction. The calli were induced at relatively higher percentage of 92% and 100% respectively in MS medium containing 2.0 mg·L^-1 2.4-D or 1.0 mg·L^-1 2,4-D and 0.1 mg·L^-1 6-BA. The embryogenic callus were induced at the MS medium containing 2.0 mg·L^-1 2,4-D or 1.0 mg·L^-1 2,4-D and 0.1 mg·L^-1 6-BA with induced rates of 3.26% and 14.52% respectively. The rates of shoot regeneration and rooting both reached 100% at the medium of 1/2 MS containing 0.01 mg·L^-1 2,4-D.
出处
《植物资源与环境学报》
CAS
CSCD
2007年第1期66-68,共3页
Journal of Plant Resources and Environment
基金
江苏省科学技术厅基础设施项目(BM2002802)
国家科学技术部"863"项目(2002AA241061)
关键词
结缕草
胚性愈伤组织
植株再生
Zoysia japonica ＇Zenith＇
embryogenic callus
plant regeneration
