摘要
目的利用 MR 显微成像技术研究阿尔茨海默病转基因小鼠老年斑的沉积情况。方法2只17个月龄阿尔茨海默病转基因[V717I]小鼠和2只野生型小鼠,行活体 T_2WI,然后对照影像定位结果进行组织学切片及免疫组织化学染色。观察 T_2WI 中老年斑的沉积情况以及其与免疫组织化学染色结果的对应关系。结果转基因小鼠 T_2WI 上显示大脑皮层和海马区可见点状低信号,且部分低信号可以和组织学切片所显示的老年斑相对应;野生型小鼠 T_2WI 未见明显的低信号,免疫组织化学染色也未见到染色阳性的斑块。结论 MR 显微成像技术检测老年斑的沉积是可行的,且可用来特异性地诊断阿尔茨海默病。
Objective MR microscopy technique was used to study the visualization of senile plaque deposition in brains of the Alzheimer disease （AD） transgenic mice. Methods Two transgenic mice and 2 wild type mice at the age of 17 months were scanned in vivo using T2 weighted image. After MR imaging, the brains were cut serially and immunostained according to the orthogonal pilot images. MR T2 weighted images and immunohistological images of the senile plaque were observed and matched. Results The MR images showed that some black spots were visible in the hippocampus and cerebral cortex of the AD transgenic mice and some spots were consistent with the senile plaques on immunohistological sections. There were no spots in the MR images and the immunohistological sections of the wild type mice. Conclusion It is possible that MR microscopy can be used to detect the deposition of the senile plaque and diagnose AD specifically.
出处
《中华放射学杂志》
CAS
CSCD
北大核心
2007年第5期538-540,共3页
Chinese Journal of Radiology
基金
国家高技术研究发展863计划(2003BA310A28)
关键词
磁共振成像
阿尔茨海默病
小鼠
转基因
Magnetic resonance imaging
Alzheimer disease
Mice,transgenic
