摘要
目的研究黄芪多糖(astragalus polysaccharide,APS)对大鼠脑出血(ICH)后血肿周围核因子-κB(nuclearfactor-kappa B,NF-κB)蛋白表达的影响,并探讨APS抗炎的脑保护机制。方法采用Ⅶ型胶原酶注射至大鼠右侧苍白球诱导ICH模型并于造模术后2 h行APS腹腔注射,分别于6 h和1、3、5 d 4个时间点进行大鼠神经行为学评分,采用免疫组化方法检测血肿周围组织NF-κB表达的动态变化,采用透射电镜观察术后3 d血肿周围神经元超微结构的变化。结果与模型组比较,APS干预后3 d和5 d大鼠神经行为学评分明显减少(P<0.05),干预6 h及1、35、d时NF-κB阳性细胞数明显减少(P<0.05),神经元超微结构改变亦比模型组减轻。结论APS可抑制NF-κB激活,减轻炎症反应,改善神经功能缺损症状。
Objective To study the protecting mechanisms of astragalus polysaccharide （APS） on the brain function and the effect of APS on inflammation after intracerebral hemorrhage （ICH）, and to investigate the expression of nuclear factor-κB （NF-κB） surround the hematoma by the intervention of APS in Sprague-Dawley male rats. Methods ICH model was induced in rats by injection of Ⅶ type collagenase into the right globus pallidus. APS was used as the intervention drug. Using immunohistochemistry we investigated the dynamic changes of the expression of NF-κB in the tissue surrounding hematoma in ICH rats. At the same time, the scores of neurological behavior in rats were recorded. Ultrastructures of the cells surround the hematoma were observed with transmission electron microscope. Results Contrast to model group, the score of neurological behavior in APS treatment group rats were decreased significantly at 3th day and 5th day （P〈0.05）, Significant difference of NF-κB positive cell between model group and treatment group occurred at 1 th day,3th day and 5th day （ P〈0.05 ）. The pathological changes of the treated group were milder than the model group on 3th day postoperative. Conclusions APS may inhibit the activation of NF-κB and effectively alleviate inflammatory response, and may accelerate the recovery of brain function interrupted by neurological deficit after ICH.
出处
《中国神经免疫学和神经病学杂志》
CAS
2007年第3期160-163,I0006,共5页
Chinese Journal of Neuroimmunology and Neurology
关键词
脑出血
核因子-ΚB
超微结构
炎症反应
intracerebral hemorrhage
NF-κB
ultrastructure
inflammatory response
