摘要
目的探讨新生鼠致痫后海马的组织病理学改变及其机制。方法对生后10d新生Wistar大鼠用戊四氮(PTZ)反复腹腔注射5d,制成反复惊厥模型,并设生理盐水对照组。采用硫堇染色观察海马神经元坏死及凋亡发生情况,对CA1、CA3、DG及门区神经元进行细胞计数;用免疫组织化学技术检测惊厥发作24h后核因子κB(NFκB)的表达;同时用Timm组织化学染色观察苔藓纤维发芽并评分。结果①海马CA1、CA3区神经元计数实验组与对照组无明显差异(P>0.05),齿状回DG区颗粒细胞数实验组为(23.25±3.06)个/250μm2,较对照组(16.25±1.58)个/250μm2增多(P<0.01)。②实验组海马CA1、CA3及DG区NFκB吸光度值较对照组高(P<0.05)。③实验组CA3区苔藓纤维发芽评分(1.50±0.92)明显高于对照组(0.25±0.46),差异有极显著性意义(P<0.01)。结论①新生鼠致痫后海马神经元无明显丢失,脑内NFκB表达增加可能对神经元起保护作用,是未成熟脑对惊厥性脑损伤具有耐受性的一种重要神经生物学基础。②新生鼠致痫后齿状回颗粒细胞增生可能是苔藓纤维发芽的启动机制。
Objective To investigate the histopathological changes of hippocampus in neonatal rats after kindling repeated seizures and the mechanism.Methods Neonatal Wistar rats with 10 postnatal days were injected intraperitoneally with pentylenetetrazol (PTZ) for 5 days to establish the repeated convulsion model (experimental group, P). The control group (C) of normal saline was set up. The necrosis and apoptosis of the neurons in hippocampus were observed and the neurons in dentate granule, CA3, CA1, as well as in hilar area were counted by thionin staining. The expression of NF-kappaB was detected by using immunohistochemical technique 24 h after kindling repeated seizures. By using Timm's method of silver sulfide staining, the mossy fiber sprouting was observed and scored.Results There was no significant difference in the cell counting of CA1, CA3 and hilar neurons between experimental group and control group (P>0.05), whereas the neurons in dentate granule were increased significantly in experimental group as compared with control group \[(23.25±3.06) vs (16.25±1.58), P<0.05\]. NF-kappaB was highly expressed in CA3, CA1 and dentate granule cells 24 h after kindling repeated seizures in experimental group as compared with that in control group (P<0.05). The mossy fiber sprouting in the CA3 was significantly increased in experimental group as compared with that in control group \[(1.50±0.92) vs (0.25±0.46), P<0.01\].Conclusion No obvious loss of cells was found in hippocampus in neonatal rats after repeated kindling. The high expression of NF-κB could be one of the important molecular basis underlying the special resistance of the neurons in premature brain to the epileptic cerebral lesions. Neurons of dentate granule were increased significantly after repeated seizures, which could be the initiative mechanism for mossy fiber sprouting in immature rats.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2005年第3期274-276,290,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
湖北省自然科学基金资助项目(No.2004ABA234)
关键词
癫痫
海马
核因子-ΚB
seizure
hippocampus
nuclear factor kappa B
