摘要
目的研究健脾方对慢性乙型肝炎(简称慢乙肝)患者树突细胞(DC)功能的影响。方法筛选慢乙肝脾虚证患者60例,随机分为治疗组和对照组各30例。对照组给予干扰素(赛诺金500万U,肌肉注射,每周3次),治疗组在此基础上加用健脾方(每日1剂,分2次服用),用药6个月。分离培养外周血DC,流式细胞仪测定DC表面HLA-DR、CD86、CD80、CD40、CD14、CD11c的表达,混合淋巴细胞反应测定两组DC的功能,治疗前后各检测1次。结果治疗组DC表面的CD86、CD80、CD40、CD11c明显高于对照组(P<0.01或P<0.05)。刺激指数、IFN-γ、IL-12的变化亦显著优于对照组(P<0.05)。结论健脾方明显改善DC的功能,调节免疫功能可能是其提高临床疗效的机理之一。
Objective To observe the effect of Spleen-invigoratlng Prescription （SIP） on dendritic cell function in patients with chronic hepatitis B. Methods A total of 60 patients with chronic HBV of Pi-deficiency syndrome type were enrolled and randomized to 2 groups, 30 in each group. Patients in the control group were given intramuscular injection with human interferon α1b, 3 times a week, while those in the treated group were given orally with SIP twice a day, the therapy lasted for 6 months. Dendritic cells （DCs） were isolated from peripheral blood and cultured, then the expression of surface markers, HLA-DR,CD86, CD80, CD40,CD14 and CD11c were detected before and after treatment by flow cytometry, and the function of DCs was also evaluated by mixed lymphocyte reaction （MLR） determination once before treatment and once after treatment. Results The expressions of DCs＇ surface CD86, CD80,CI40 and CDllc in the treated group were higher （P 〈0.05, P〈0.01） and the changes of stimulating index , IFN-γ and IL-12 were superior in the treated group to those in the control group （P 〈 0.05）. Conclusions SIP can significantly improve DC＇ s function, so, one of mechanisms of SIP in improving clinical efficacy may be the regulation of immune function.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2007年第4期300-302,共3页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家中医药管理局科学技术研究资助项目(NoGZJZ-T20040312)
上海市重点学科建设项目资助(NoY0302)
上海市教育委员会E-研究院建设计划项目资助(NoE03008)
关键词
慢性乙型肝炎
树突细胞
脾虚
健脾方
chronic hepatitis B
dendritic cells
Pi-deficiency
Spleen-invigorating Prescription
