摘要
目的探讨Tca8113细胞长期接触卡铂以后耐药性表达情况。方法以Tca8113细胞系为研究对象,采用间歇性加药,逐步递增剂量,体外连续培养诱导其产生耐药性。用MTT法检测细胞耐药指数,LsAB免疫组化法检测耐药蛋白P糖蛋白-170(Pgp-170)、谷胱苷肽S转移酶-π(GST-π)、多药抗药性相关蛋白(MRP)和拓扑异构酶Ⅱ(TopoⅡ)的表达,RT-PCR检测多药耐药基因(MDR)、MRP和GST-π的表达。结果诱导后Tca8113/卡铂(CBP)对氨甲喋呤(MTX)、CBP、平阳霉素(PYM)、长春新碱(VCR)的抗药性明显升高,但对5-氟尿嘧啶(5-FU)抗药性增加不明显。在免疫组化和RT-PCR中Pgp-170、MRP和GST-π表达升高,TopoⅡ表达降低。结论Tca8113细胞系在卡铂长期刺激的环境中多种耐药相关蛋白表达上升,表明肿瘤细胞的耐药现象受到多种基因的调节。Tca8113/CBP可以作为一个肿瘤耐药研究的平台。
Objective To study the drug resistance changes in Tca8113 cell lines by exposing to carboplatin. Methods The concentration of carboplatin added to Tca8113 ceils was increased gradually and continually, which was to induce the carboplatin-resistance in Tca8113 ceils. The sensibility to drugs of the ceils was analyzed by MTY method. Immunocytochemistry and RT-PCR were utilized to examine the expression of multidrug resistance proteins and genes. Results After exposing to carboplatin, the Tca8113/CBP ceils had higher drug-resistance to CBP, MTX, PYM, VCR and higher expression of MRP, GST-π than Tca8113 ceils. Conclusion Muhidrug resistance of Tca8113/CBP is associated with over expression of MRP, GST-π and MDR. Tca8113/CBP can provide an ideal modal for multidrug resistance research.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2007年第2期184-187,共4页
West China Journal of Stomatology
基金
国家自然科学基金资助项目(39970798)
四川大学优秀博士论文研究基金资助项目(0040305505010)
