摘要
目的:探讨人舌鳞癌细胞荷瘤裸鼠体内化疗药物持续刺激状态下,肿瘤的生长及其耐药性的变化情况。方法:采用BALB/c-nu/nu裸鼠皮下接种Tca8113细胞悬液,待裸鼠成瘤后,采用卡铂(Carboplatin,CBP)进行小剂量长期暴露法诱导肿瘤耐药,以观察体内肿瘤耐药性的产生、肿瘤的生长变化及其各种耐药蛋白的表达。用免疫组化法检测部分耐药蛋白表达情况,RT-PCR检测相关耐药基因表达情况。结果:诱导后Tca8113/卡铂在免疫组化和RT-PCR检测中,MRP、GST-π等表达升高,TopoⅡ表达降低。结论:利用荷瘤裸鼠体内给药诱导肿瘤耐药性的产生可以更好地模拟临床舌鳞癌MDR的发生发展过程及其生物学特性,为多药耐药性及其逆转的进一步研究提供了一个有价值的研究平台。
Objective:To study the muhidrug resistance phenotype in nude mice bearing tumors of Tca8113 cell by espousing to Carboplatin.Method:The Tca8113 cells were injected subcutaneously in both sides of armpit of nude mice with the concentration of 5×10^6/0.1 ml.Two weeks after injection Carboplatin was used subcutaneously around the tumor 10 mg/g(weight)each day in Tca8113/CBP group while Tca8113 group was injected with physiologic saline.Mice were sacrificed about 13 weeks after drug inducing.The tumors were examined by Immunocytochemistry,RT-PCR et,al.Result:After espousing to Carboplatin,MDR of Tca8113/CBP group was associated with over expression of MDR,MRP,GST-πboth in protein and gene levels than Tca8113 group.Conclusion:The study showed MDR phenotype was elevated obviously in the inducing group,and in vivo inducing MDR was a good way to establish investigation platform for MDR mechanisms and reversing study.
作者
冯戈
王大章
陈槐卿
何嘉
冷卫东
FENG Ge;WANG Da-zhang;CHEN Huai-qing;HE Jia;LENG Wei-dong(Key Laboratory of Oral Biomedical Engineering Ministry of Education,Sichuan University,Chengdu 610041,China)
出处
《临床口腔医学杂志》
2007年第2期78-81,共4页
Journal of Clinical Stomatology
基金
国家自然科学基金资助项目(39970798)
四川大学优秀博士论文研究基金资助项目(0040305505010)
关键词
TEA8113细胞
多药耐药性
卡铂
裸鼠
Tca8113 cell line
muhidrug resistance
carboplatin(CBP)
nude mice
