摘要
为研究金银花不同采收期和不同部位提取液膜保护酶的活性与清除自由基的能力,采用生物化学分析法测定了不同采集期金银花花蕾、叶片和枝条膜保护酶活性,结果显示,SOD、POD和CAT活性从4月开始平稳上升,6月上旬前后达到最高值。叶片的上述指标最高,枝条最低。对自由基的清除能力也呈平稳上升趋势。金银花花蕾对超氧自由基和羟自由基的清除效应最高,花蕾和叶片对过氧化氢清除效应相当,枝条的最低。几种膜保护酶活性之间均呈较显著的正相关,当膜保护酶活性增强时,清除自由基的能力也随之提高,可能是它们的协调作用使金银花有较强的清除自由基的能力。
In order to study the activity of membrane-protecting enzyme extracted from Lonicera japonica Thunb and the scavenging capability on free radicals, the biochemical method was used to analyse the activity of membrane-protecging enzyme which was extracted from flower bud, leaf and wattle of Lonicera japonica thunb in different harvest time. The analytical results showed that the activity of the enzyme was increased since April and reached the highest level in June. The activity of the enzyme extracted from Lonicera japonica Thunb leaf was higher than those from its flower bud and wattle. The scavenging capacity of the extract from the flower bud on ·OH and H2O2 free radicals was stronger than those from leaf and wattle. The activity of enzyme extracted from Lonicera japonica Thunb varies from April to June, and was highly relative to the scavenging capability on free radicals.
出处
《中国食品学报》
EI
CAS
CSCD
2007年第2期27-32,共6页
Journal of Chinese Institute Of Food Science and Technology
关键词
金银花
酶活性
自由基
清除能力
生物化学
Lonicera japonica Thunb Enzyme activity Free radicals Scavenging capacities Biochemistry
