摘要
分析乌骨鸡正己烷提取物组成成分,并研究其抗皮肤衰老活性。采用GC/MS联用技术和HPLC法分别测定乌骨鸡和麻鸡正己烷提取物中的脂肪酸组成和α-维生素E含量。每日早晚分别在老龄小鼠脱毛处涂抹甘油(Ⅰ)、乌骨鸡正己烷提取物(Ⅱ)、麻鸡正己烷提取物(Ⅲ)各一次。14d后处死小鼠测定皮肤中含水量、羟脯氨酸及胶原蛋白含量。成分分析表明Ⅱ中多不饱和脂肪酸、必需脂肪酸、花生四烯酸及α-维生素E含量均显著高于Ⅲ。与对照组Ⅰ比较,Ⅱ和Ⅲ组均可提高小鼠皮肤含水量(P<0.01);Ⅱ组可显著提高小鼠皮肤羟脯氨酸和胶原蛋白含量(P<0.01),提示Ⅱ外用有抗皮肤衰老活性,其抗皮肤衰老活性可能与富含维生素E和多不饱和脂肪酸有关。
The composition and anti-aging activity of hexane extracts from Black-bone Silky fowl were investigated. The fatty acid composition and α-tecopherol content in hexane extracts from Black-bone silky fowl and Chongren chicken were determined by GC/MS and HPLC respectively. Three groups of the healthy old KM mice which were shaved on the back skin were smeared with glycerol （ Ⅰ ）, hexane extracts from Black-bone silky fowl （ Ⅱ ）and hexane extracts from Chongren chicken（ Ⅲ ） twice a day, respectively. The contents of water, hydroxyproline （Hyp） and collagen in derma were determined after 14 days. The results showed that Ⅱ had higher contents of polyunsaturated fatty acids （PUFAs）, essential fatty acids （EFAs） ,arachidonic acid （AA） and α-tecopherol than those in group m. Group Ⅱ increased the water,Hyp and collagen contents in derma of old KM mice compared with Ⅰ （P 〈 0.01 ）. It suggested that Ⅱ has good anti-aging effects on mice skin, which may have correlation with higher contents of α-tecopherol and PUFAs.
出处
《天然产物研究与开发》
CAS
CSCD
2007年第2期225-228,273,共5页
Natural Product Research and Development
基金
教育部长江学者和创新团队发展计划(IRT0540)
江西省农业科技重点攻关项目(2005)
南昌大学测试基金项目(2005029)
