摘要
利用ST细胞研究了甘草酸对猪呼吸道冠状病毒(PRCV)体外复制过程中的抑制作用。通过RT-PCR和Western blot检测发现甘草酸能有效抑制PRCV的体外复制而对ST细胞不引起明显细胞毒作用。抑制效果与甘草酸的剂量成正比关系。PRCV病毒核衣壳蛋白的表达无论在mRNA水平还是在蛋白质水平均随着甘草酸添加量的增加而降低,提示甘草酸是一种有效的抗PRCV药物。
The inhibitory effect of glycyrrhizin （GL） on porcine respiratory coronavirus （PRCV） replication was investigated in vitro in ST cells using RT-PCR and Western blot techniques. GL effectively suppressed PRCV replication without causing apparent cell cytotoxicity in ST cells. The inhibitory effect of GL on PRCV was in a dose-dependent manner. The expression levels of both mRNA and protein of the PRCV nucleocapsid protein in ST cells were impeded by the increasing addition of GL,suggesting it is a potential effective antiviral component against PRCV in vitro.
出处
《天然产物研究与开发》
CAS
CSCD
2007年第2期221-224,共4页
Natural Product Research and Development
基金
This project was supported by a grant from the Bureauof Science and Technology of Fujian Province (2003N083)
agrant from the Bureau of Science and Technology of Xiamen City(3502Z20031054)
关键词
甘草酸
核衣壳蛋白
猪呼吸道冠状病毒
glycyrrhizin
nucleocapsid protein
porcine respiratory coronavirus （PRCV）
