摘要
对接种后水培15天的大豆幼苗的根段染色,光镜下可以观察到抗病品种根内大豆孢囊线虫头部有明显的坏死,感病品种较少观察到这一现象。接种后4天对大豆根部的半薄切片研究发现感病品种中柱内有较大的合胞体形成,抗病品种在鞘细胞处形成较小的合胞体,且染色较深。
The inoculated soybean seedlings had been hydroponic cultured for 15 days, and the roots were removed and stained. Necrosis adjacent to the head of second stage and third stage juveniles of Hetecdera glycines in the roots of resistant cultivars Huipizhiheidou and Yuanboheidou was found under light microscope, whereas, necrosis was not found in the roots of susceptible control, Ludou 1. Semi-thin sections of infected roots cultured in sterilized sand were inspected, and the bigger synvytia in central cylinder were found in susceptible roots, whereas the smaller syncytia, which were stained deeply, were observed on pericycle cells in resistant roots at 4 days after inoculation.
出处
《大豆科学》
CAS
CSCD
北大核心
1997年第1期34-37,共4页
Soybean Science
关键词
大豆
抗源
孢囊线虫
组织坏死
组织病理学
Resistant soybean varieties
Heterodera glycines
Histopathology
Nevrosis