阿托伐他汀对大鼠心肌梗死后心肌间质转化生长因子-β1表达的干预研究被引量：2

The interventional effect of atorvastatin on expression of transforming growth factor -betal of myocardial extracellular matrix after infarction in the rats

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摘要目的探讨大鼠心肌梗死后心肌间质转化生长因子-β1（TGF-β1）的表达及阿托伐他汀对其的影响。方法结扎SD大鼠冠状动脉前降支复制急性心肌梗死模型,随机分成心肌梗死对照组（n=26）和阿托伐他汀治疗组（n=27）。两组又随机分为1周、2周和4周亚组。另设假手术组（n=8）。用逆转录-聚合酶链反应法（RT-PCR）及免疫组化方法检测TGF-β1 mRNA和蛋白表达情况。结果与假手术组相比,心肌梗死对照组TGF-β1的mRNA表达在各亚组明显升高（均P〈0.05）,1周和2周亚组蛋白表达也均增高（均P〈0.05）。而阿托伐他汀治疗组TGF-β1蛋白表达在1周和2周亚组,mRNA的表达在1周、2周、4周亚组,均较心肌梗死对照组显著降低（均P〈0.05）。结论阿托伐他汀可抑制心肌梗死后心肌间质TGF-β1的表达,可能会间接改善心肌梗死后心室重塑的发生发展过程。 Objective To investigate the effect of atorvastatin on expression of transforming growth factor - betal （ TGF - β1 ） of myocardial extracellular matrix after infarction in the rats. Methods One hundred and ten adult male Sprague - Dawley rats underwent the left descending coronary artery ligation , then being divided randomly into AMI control group （n = 26）and AMI atorvastatin treatment group （n = 27）. The rats in those two groups were redivided into 3 sub - groups according to the course of treatment ： 1 - week, 2 - week, 4 - week sub - groups. Eight rats underwent sham operation, being killed after 4 weeks. The mRNA and protein expression of TGF - β1 was examined by reverse transcription - polymerase chain reaction （ RT - PCR） and immunohistochemistry. Results Compared to sham operated rats, the mRNA expression of TGF - β1 showed significant increase in all sub- groups of AMI controls（ all P 〈0.05）, and the protein expression of TGF - β1 were enhanced in the 1 - week and 2 - week sub - groups （ all P 〈 0.05 ） . In comparison with the AMI control group, the protein expression of TGF - β1 were significantly lower in the 1 - week and 2 - week sub- groups of atorvastatin group（all P 〈0.05） and so were the mRNA expression of TGF- β1 in the 1 - week,2 - week,4 - week sub - groups （ all P 〈 0.05 ）. Conclusion The expression of TGF - β1 in the myocardial extracellular matrix increased after infarction. These changes suggest that TGF - β1 plays an impor- tant role in postinfarction extracellular matix remodeling. Atorvastatin probably can prevent ventricular remodeling by suppressing the enhanced expression of TGF - β1.

作者陈昭喆宋亚辉谢秀乐袁俊强孙向东

机构地区河南省信阳市中心医院心内科

出处《中原医刊》 2007年第7期26-28,共3页 Central Plains Medical Journal

关键词转化生长因子-Β1 心肌梗死心室重塑阿托伐他汀 Transforming growth factor - betal Myocardial infarction Ventricular remodeling Atorvastatin

分类号 R542 [医药卫生—心血管疾病]

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