摘要
The binding characteristics of neutral red （NR） with DNA were investigated by fluorescence spectrometry. Chemometrics approach as singular value decomposition （SVD） was used to evaluate the number of spectral species in the drug-DNA binding process, and then the intrinsic binding constant of 1.6 × 10^4 in base pairs and the binding site number of 0.97 were obtained from the Scatchard plot.
The binding characteristics of neutral red （NR） with DNA were investigated by fluorescence spectrometry. Chemometrics approach as singular value decomposition （SVD） was used to evaluate the number of spectral species in the drug-DNA binding process, and then the intrinsic binding constant of 1.6 × 10^4 in base pairs and the binding site number of 0.97 were obtained from the Scatchard plot.
基金
The financial support from the National Natural Science Foundation of China （No. 20562009）；the Natural Science Foundation of Jiangxi Province （No. 0620041）；the foundation of the State Key Laboratories of the Chemo/ Biosensing and Chemometrics of Hunan University （No. 2005-22）；the program for Changjiang Scholars and Innovative Research Team in Universities （No. IRT0540）.
