摘要
以柞蚕蛹为原料,经冷冻干燥、石油醚脱脂处理后,得到脱脂柞蚕蛹粉;用定磷法测得脱脂柞蚕蛹粉中核酸的含量为:DNA的含量为1.25%,RNA的含量为2.37%,RNA与DNA之比约为2:1,总的核酸含量为3%～4%;用浓盐法从脱脂柞蚕蛹粉中提取DNA,最佳提取条件为:食盐浓度10%,时间为3h,固液比1:6,在此条件下,蛹粉DNA的得率为0.62%～0.66%。
In this paper ,tussah pupa was used as experimental material ,the de-fating tussah pupa powder was obtained by freeze-drying and de-fating by petroleum ether.The nucleic acid content in the de-fating tussah pupa powder that is determined by fixing phosphorus method is listed as follows：the content of DNA is 1.25 %,the content of RNA is 2.37 %,the ratio between RNA and DNA is 2 ： 1,the total content of nucleic acid is 3 %-4 %;DNA is extracted by strong salt method from de-fating tussah pupa powder,the optimum extraction conditions are obtained as follows ：the concentration of salt is 10 % for 3 h,the ratio of material to solvent is 1 ： 6,under such conditions,the yield of pupa DNA is0.62 %-0.66 %.
出处
《食品研究与开发》
CAS
北大核心
2007年第4期87-90,共4页
Food Research and Development
关键词
柞蚕蛹
脱氧核糖核酸
测定
提取
tussah pupa
deoxyribonucleic acid
determination
extraction
