摘要
采用大花蕙兰的试管苗和原球茎作材料。以MS培养基或1/2MS为基本培养基,比较不同浓度的队对大花蕙兰试管苗和原球茎增殖的影响,以及不同浓度的NAA对大花蕙兰试管苗生根的影响。试验结果表明:大花蕙兰原球茎增殖与分化的最佳培养基为(1/2MS+NAA 0.2mg/L+B.A1.0 mg/L),原球茎增殖率达363.16%,芽分化率达到68.42%;其试管苗增殖的最佳培养基为(MS+NAA0.2mg/L+香蕉汁100g/L+BA0.5mg/L),芽增殖率达到91.67%;大花蕙兰试管苗生根的最佳培养基为(1/2MS+NAA0.5mg/L),平均每株生根数为2.60条。
The materials were test-tube plantlet and protocorm of cymbidium grandiflorium. Basic Culture Mediumare were MS or 1/2MS,Comparing the effect of different concentration of BA on test-tube plantlet and protocorm multiplicating of cymbidium grandiflorium,the effect of differem concentration of NAA on test-tube plantlet rooting of cymbidium grandiflorium. The experiment results indicated： 1/2MS＋NAA0. 2mg/L＋BA1. 0 mg/L was the best Culture Mediumare of multiplication and differententiation of protocorm of cymbidium grandiflorium,The ratio of multiplication of protocorm was 363. 16% and differentiation of bud was 68. 42%. The best Culture Mediumare of test-tube plantlet multiplicating was MS＋NAA0. 2mg/L＋ Banana Juice 100g/L＋ BA0. 5mg/L, The ratio of multiplication of bud was 91.67% ;The best Culture Mediumare of test-tube plantlet roofing was 1/2MS＋NAA0. 5mg/L, the average number of rooting was 2. 60%.
出处
《北方园艺》
CAS
北大核心
2007年第4期218-219,共2页
Northern Horticulture
关键词
大花蕙兰
组织培养
分化
生根
Cymbidium grandiflorium
Tissue
Differentiation
Culture-rooting
