摘要
通过单因素和正交实验对纤维素酶提取枸杞粗多糖的工艺条件进行优化,结果表明:最佳酶解工艺条件为纤维素酶用量0.45%、pH为4.5、酶解温度50℃、酶解时间160min,在此优化条件下,提取枸杞粗多糖的得率为23.68%,与热水法浸提的粗多糖8.65%相比有明显提高。其中采用酶法提取的粗多糖中的枸杞多糖含量也提高到了31.3%,为热水浸提法所得枸杞多糖含量的1.8倍。
On basis of single factor and orthogonal experiments, extraction of Lycium barbarum polysaccharides by cellulase was investigated. The results showed that the optimal enzymolysis conditions were as follows： the amounts of cellulose was 0.45%, the pH of extracting media was 4.5, the enzymolysis temperature was 50~C and the enzymolysis time was 160min. In optimal conditions, the yield of crude polysaccharides of Lycium barbarum extracted by cellulose was 23.68%, which had an obvious increase compared with 8.65% of crude polysaccharides extracted with hot water. And the content of Lycium barbarum polysaccharides by enzymolysis was 31.3%, which was 1.8 times the content of Lycium barbarum polysaccharides extracted with hot water.
出处
《食品科技》
CAS
北大核心
2007年第3期104-106,共3页
Food Science and Technology
关键词
纤维素酶
构杞
多糖
cellulose
Lycium barbarum
polysaccharids
