摘要
本文采用DNA聚合酶α或/和β酶专一性抑制剂(NEM或/和d_2TTP),观察了小鼠腹水型H_(22)肝癌细胞核DNA聚合酶α、β在40Gy γ线照后DNA修复合成中的作用。结果表明:在α酶被完全抑制条件下,照射组在37℃保温60、120min后DNA修复合成均明显高于未受照组(P<0.05、P<0.01);当β酶被完全抑制条件下,照射组与未受照组在37℃保温30、60和120min后DNA修复合成无明显差别(P>0.05);当α及β酶均被抑制时,照射组与未受照组DNA修复合成亦无明显差别(P>0.05)。上述结果证明了在本实验条件下,β酶参与了受γ线照射后肝癌细胞核DNA的修复作用,而α酶则不参与。作者并对上述结果进行了讨论。
The effects of DNA polymerase alpha and beta on DNA repair of the40 Gy γ-rays irradiated H_(22) cell nuclei, using specific inhibitor NEM or ddTTP toDNA polymerase alpha or beta respectively, were observed. The results showed thatthe rate of DNA repair synthesis of the 40 Gy γ-rays irradiated H_(22) cell nuclei wasmuch higher than that of the non-irradiated ones after 60, 120 min incubation at 37℃(P<0. 05,P<0. 01)when DNA polymerase alpha was inhibited completely. There wasno difference in the rate of DNA repair synthesis between the irradiated and the non-irradiated cell nuclei (P>0. 05) when DNA polymerase beta was inhibited completely.And also, there was no difference between them (P>0. 05) when both DNA polyme-rase alpha and beta were inhibited completely. These results indicate that under ourexperimental conditions, DNA polymerase beta, not alpha, is responsible for DNArepair of the γ-rays irradiated H_(22) cell nuclei. The above results are discussed by theauthor considerably.
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
北大核心
1990年第3期150-154,144,共6页
Journal of Radiation Research and Radiation Processing
基金
国家自然科学基金
关键词
DNA
聚合酶
修复
肝癌
DNA polymerase alpha
DNA polymerase beta DNA repair
NEM
ddTTP
