摘要
目的探讨MMP-9、TIMP-1及细菌L型在卵巢上皮性肿瘤中的表达及临床意义。方法采用原位杂交和免疫组化及革兰染色方法检测97例卵巢乳头状癌及23例卵巢乳头状瘤组织中MMP-9、TIMP-1的表达及细菌L型检出率，并用r检验进行统计学处理。结果卵巢乳头状癌中MMP-9及TIMP-1的表达率均明显高于良性肿瘤（P〈0．005）。MMP-9在卵巢乳头状癌中临床分期Ⅲ、Ⅳ期中的表达率明显高于Ⅰ、Ⅱ期（P〈0．005-P〈0．01），随着病理分级增高而显著增加（P〈0．005～P〈0．05），腹腔淋巴结有转移和有腹水者均高于无腹腔淋巴结转移和无腹水者（P〈0．005～P〈0．05）。而TIMP-1阳性表达与MMP-9阳性表达相反，呈负相关。细菌L型检出阳性率与病理分级及临床分期差异有显著性，腹腔淋巴结有转移比无转移者、有腹水比无腹水者差异有显著性（P〈0．005）。结论MMP-9、TIMP-1基因及蛋白在卵巢肿瘤中有不同程度的异常表达，两者均可作为判断卵巢肿瘤生物学行为及患者预后参考指标。L型感染极有可能成为诱发肿瘤因素之一，它与MMP-9、TIMP-1可能有协同致瘤及恶性肿瘤侵袭和转移作用。研究细菌L型感染与肿瘤的关系，具有重要的临床应用价值。
Objective To study the expression of MMP-9 ,TIMP-1 and infection of bacterial L-froms in ovarian neoplasms and to discuss the clinical significance. Methods Hybridization in situ and immunohistochemical staining was used to detect the expression of MMP-9,TIMP-1 mRNA and protein in 120 cases of ovarian tumors. Gram＇ s straining was used to examine the positive rate of bacterial L-forms in these tissues. Results The positive rates of MMP-9 ,TIMP-1 mRNA and protein expression was higher in malignant tumors than that in benign ones（P 〈0.005）. The expression of MMP-9 and TIMP-1 were higher in clinical stage Ⅰ , Ⅱ than that in stage Ⅲ, Ⅳ ,and which was increased with the grade（P 〈0. 005 to P 〈0.01 ）. The expression of these two marks were higher in patients with lymph node involvement and ascites than those without that （ P 〈 0. 005 to P 〈 0.05 ）. The positive expression of TIMP- 1 was opposite to MMP-9 （ P 〈 0. 005 - P 〈 0. 05 ）. The positive rate of bacterial L-froms was no high in malignant tumors than that in benign ones（ P 〉 0.05 ） ,but it was increased with clinical stage and pathological grade （ P 〈 0. 005 ）. The expression of L-froms was higher in patients with lymph node involvement and ascites than those without that（ P 〈 0.005 ）. Conclusions In ovarian neoplasma,the abnormal expression of MMP-9 ,TIMP-1 and positive rate of bacterial L-forms have obvious relationship with clinical stage, pathological grade,lymph node involvement and ascites. Bacterial L-forms maybe one of the inductive factors of the occurrence of tumor. The expression of MMP-9 and TIMP-1 with cooperative of L-forms is likely to promote a faster invasion and metastasis of ovarian carcinomas. Therefore, to study the infection of bacterial L-forms has an important clinical significance.
出处
《中国微生态学杂志》
CAS
CSCD
2007年第2期173-176,共4页
Chinese Journal of Microecology
基金
安徽省教育厅资助项目(2002kj226)
关键词
卵巢肿瘤
MMP-9
TIMP-1
原位杂交
免疫组化
细菌L型
Ovarian neoplasma
Matrix metalloproteinase-9
Tissue inhibitor of metalloproteinase-1
Immunohistochemical
Hybridization in situ
Bacterial L-forms
