摘要
证实过氧钒烟酸络合物（ＰＯＲ）在链脲佐霉素糖尿病大鼠体内的降糖和促进葡萄糖载体转位的作用。方法ＰＯＲ在实验室条件下合成，并用质谱和红外鉴定其结构。ＰＯＲ、钒酸钠用其水溶液通过灌胃的方法给予。经处理的糖尿病大鼠的骨骼肌用于制备微粒体膜（ＭＭ）和浆膜（ＰＭ）。葡萄糖载体ＣＯＯＨ端１６肽抗血清用于ＷｅｓｔｅｒｎＢｌｏｔ估计载体的转位。结果ＰＯＲ有明显的降糖作用。１ｍｇ／ｋｇ的ＰＯＲ在４周末使糖尿病大鼠血糖降至接近正常水平，而同等剂量的钒酸钠、烟酸并不表现出明显的降糖作用。ＷｅｓｔｅｒｎＢｌｏｔ的结果：浆膜葡萄糖载体Ｇｌｕｔ４、Ｇｌｕｔ１的水平比微粒体葡萄糖载体Ｇｌｕｔ４、Ｇｌｕｔｌ的水平均明显升高（Ｐ均＜０．０１）；ＰＯＲ处理组浆膜Ｇｌｕｔ４、Ｇｌｕｔ１的水平比对照组明显升高（Ｐ均＜０．０１）；提示ＰＯＲ可促进Ｇｌｕｔ４、Ｇｌｕｔ１的转位。结论过氧钒烟酸络合物ＰＯＲ具有降糖作用。
Objective To demonstrate the hypoglycemic effects and translocation of glucose transportor(Glut 1and Glut4)promoted by peroxovanadate and nicotinic acid complexes(POR)in streptozotozin induced diabetic rats.Methods Peroxovanadate complexes nicotinic acid(POR)was prepared in laboratory.POR and vanadate were administered in drink water.The muscles from diabetic rats were subjected to sucro sedensity gradient centrifugation to prepare plasma membrane and microsome membrane.Antibodies to COOH terminal of glucose transportor were used in Western Blot to evaluate the translocation.Results Peroxovanadate complexes of nicotinic acid(POR)showed marked hypoglycemic effects on STZ induced diabetic rats.1mg/kg oral pathway POR could significantly reduce the plasma glucose levels(from 18.95±2.61 mmol/L to 6.36±2.23 mmol/L, t =12.233, P <0.01)over four week's treatmen t,whereas,same dose of single sodium vanadate or nicotinic acid did not have hypoglycemic effects.Thenet vanadium intake was about1/90o fsingle effectively vanadate treatment.When Western blot was used POR increased the translocation of Glut4and Glut1from intracellular site of plasma membran e.Conclusion Peroxovanadate nicotinic acid complexes(POR)are the novel vanadyl that markedly reduce plasma glucose in a lower dose comparing to vanadate in STZ DM rats by oral administration.Translocation of glucose transportor may play apart in hypoglycemic mechanism.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1997年第3期208-211,共4页
National Medical Journal of China
基金
国家教委博士点科研专向基金
