摘要
目的研究骨巨细胞瘤组织中基质金属蛋白酶-2(Matrix metalloproteinase-2)及其特异性抑制剂金属蛋白酶组织抑制因子-2(Tissue inhibitor of metalloproteinase-2)的表达与肿瘤血管形成和关系。方法用免疫组化SP法检测45例骨巨细胞瘤组织中MMP-2、TIMP-2的表达情况,以CD31标记检测微血管密度(MVD),计算肿瘤复发组和非复发组MMP-2/TIMP-2的比值,分析这些指标与骨巨细胞瘤预后的关系。结果骨巨细胞瘤组织有高水平的MMP-2、TIMP-2和CD31表达。复发组表达MMP-2、MMP-2/TIMP-2比值以及MVD均显著高于非复发组,但与组织学分级无关。MMP-2与MVD呈正的直线相关。结论MMP-2/TIMP-2比值升高与骨巨细胞瘤的复发以及肿瘤血管形成有关。
Objective To investigate the relationship between expression of matrix metalloproteinase- 2 （MMP- 2）, tissue inhibitors of metalloproteinase- 2 （TIMP- 2） and angiogenesis and recurrence of human giant cell tumor of bone. Methods Expression of MMP - 2, TIMP- 2, and CD31 in 45 patients with giant cell tumor of bone were detected by immunohistochemistry. The ratio of MMP - 2/TIMP - 2 was calculated and used as an indicator in comparison of recurrent rate of tile tumor. Results Over - expression of MMP - 2, TIMP- 2, and micro vessel density （MVD） in giant cell tumors of bone was observed. The expression levels of MMP- 2, ratio of MMP- 2/TIMP- 2 and MVD were significantly higher in recurrence group than non- recurrence group of the tumor. However, there was no relationship between expressions of MMP - 2, TIMP- 2, MVD and histological grading of the tumor. MMP - 2 was positively correlated with MVD. Conclusion The recurrence of giant cell tumor of bone is correlated with high expression of MMP - 2, MVD and high ratio of MMP - 2/TIMP - 2.
出处
《中国热带医学》
CAS
2007年第5期685-686,共2页
China Tropical Medicine
基金
广东省自然科学基金项目(No.001315)
关键词
骨巨细胞瘤
基质金属蛋白酶
金属蛋白酶组织抑制物
微血管密度
Giant cell tumor of bone （GCT）
Matrix metalloproteinase - 2 （MMP - 2）
Tissue inhibitor of metalloproteinase - 2 （TIMP-2）
Micro vessel density （MVD）
