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日本曲霉β-呋喃果糖苷酶基因克隆及在酿酒酵母中的表达 被引量：2

Cloning and Expression of β-Fructofuranosidase cDNA from Aspergillus japonicus in Saccharomyces cerevisiae
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摘要 通过反转录PCR法成功地扩增出日本曲霉β-呋喃果糖苷酶基因,基因片断长度为1 917bp,编码638个氨基酸。将所得片断定向克隆到pYX212载体上,并转化至酿酒酵母中,通过酵母菌落PCR检验后,确定该基因在酿酒酵母中获得表达,转化子平均酶活为26.4 U/mg。 A β-fructofuranosidase gene of 1 917 bp was cloned from Aspergillus japonicus, and the ORF sequence predicted a protein of 638 amino acids. A expression plasmid pYX212 carrying the gene was under the control of the triose phosphate isomerase gene （TPI） promotor was transformed into Saccharomyces cerevisiae W303-1A, and β-fructofuranosidase gene was successfully expressed in the recombinant strains. The enzyme activity was about 26.4 U/mgo
作者 王立梅 齐斌 周惠明
机构地区 常熟理工学院生物与食品工程系 江南大学食品学院
出处 《食品与生物技术学报》 CAS CSCD 北大核心 2007年第2期62-66,共5页 Journal of Food Science and Biotechnology
基金 国家自然科学基金项目(30471155)
关键词 日本曲霉 Β-呋喃果糖苷酶 CDNA克隆 酿酒酵母 Aspergillus japonicus β-fruetofuranosidase eDNA clone Saccharomyces cerevisiae
分类号 Q786 [生物学—分子生物学]
  • 相关文献

参考文献11

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共引文献36

同被引文献50

  • 1童群义,朱桂兰.节杆菌β-呋喃果糖苷酶的纯化及性质研究[J].食品科学,2005,26(3):69-71. 被引量：6
  • 2屈慧鸽,李荣,缪静,张立,刘进杰.果胶酶对红葡萄酒主要成分的影响[J].酿酒科技,2005(8):71-73. 被引量：15
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引证文献2

二级引证文献9

食品与生物技术学报
2007年 第2期

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