摘要
为筛选得到高产琥珀酸的厌氧菌株,建立了一种快速半定量分析发酵液中琥珀酸的方法。首先通过高效液相色谱法(HPLC)分析出产琥珀酸厌氧菌发酵液中主要含有琥珀酸,乳酸和乙酸,然后将发酵液经过纸层析展开后,发现琥珀酸可以和副产物乳酸,乙酸完全分开,最后用半定量的方法求出琥珀酸的含量。结果表明纸层析法简单经济,可以作为产琥珀酸厌氧菌的初筛方法。
In order to obtain an efficient screening procedure for identification of succinate producing anaerobic strains, a semi-quantitative paper chromatography method was developed. Lactic acid and acetic acid were identified as the main byproducts in the process of succinate production by the high performance liquid chromatography （HPLC） . Succinic acid was completely separated from the byproducts of lactic acid and acetic acid in the same broth developed by paper chromatography. The content of succinic acid was calculated by a semi-quantitative method. The results showed that paper chromatography was a simple and cost effective method that could be utilized to screen anaerobic strains producing succinic acid.
出处
《微生物学通报》
CAS
CSCD
北大核心
2007年第2期315-318,共4页
Microbiology China
基金
无锡市"学术技术带头人培养工程"资助项目(No200502)
关键词
琥珀酸
厌氧微生物
纸层析
高效液相色谱
Succinic acid, Anaerobic organisms, Paper chromatography, HPLC
