摘要
目的建立盐酸帕洛诺司琼注射液含量测定的高效液相色谱方法。方法采用Alltima C18色谱柱（4．6mm×250mm，5μm）。以0．02mol·L^-1磷酸二氢钠溶液（含0．25％的三乙胺与0．01mol·L^-1己烷磺酸钠，用磷酸调节pH值至3．0）-乙腈（40：60）为流动相；检测波长为254nm，流速为1．5mL·min^-1。结果线性范围为10．3～103．1mg·L^-1，r=0．9999；平均回收率为100．4％。RSD为0．95％（n=9）。结论本方法简便、可靠，可用于盐酸帕洛诺司琼注射液的含量测定。
OBJECTIVE To establish a HPLC method for the determination of palonosetron hydrochloride for injection. METHODS The separation was performed on a Alhima C18 column（4. 6 mm × 250 mm,5 μm）with the mobile phase consisting of 0. 02 mol·L^-1 sodium dihydrogen phosphate（contain 0. 25% triethylamine and 0. 01 mol·L^-1 sodium hexanesulfonate,adjust the pH to 3.0 with phosphoric acid） -acetontrile（40： 60）. The detection wavelength was 254 nm. RESULTS The method was proved to be linear in the range of 10. 3 - 103.1 mg·L^-1 ,the regression coefficient was 0. 999 9. The recovery of method was 100.4% （ RSD 0. 95% ,n = 9）. CONCLUSION The method is simple, reliable and suitable for the determination of palonosetron hydrochloride for injection.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2007年第6期473-475,共3页
Chinese Pharmaceutical Journal
