摘要
目的:研究大鼠血管内膜损伤后血管重塑的相关因子,包括基质金属蛋白酶(MMP-1,MMP-2),金属蛋白酶组织抑制物(TIMP-2),转化生长因子-1β(TGF-1β)的变化规律。方法:建立大鼠胸主动脉球囊拉伤模型,分为对照组,术后3d、5d、8d、14d、21d和35d组。采用免疫组化方法检测各因子的表达。结果:动脉内膜损伤后3d各因子均明显表达增加,随后下降,TGF-β1至术后8d,MMP-1至术后14d,MMP-2至术后21d降低至与对照组无区别,TIMP-2至术后21d仍持续高于对照。结论:大鼠血管损伤后修复过程中各因子作用时间窗不完全相同,动脉球囊拉伤术后3周内可能是药物干预的最佳时机。
Objectlve：To observe the changes of matrix metalloproteinases （MMP-1,MMP-2）,tissue inhibitors of metalloproteinase-2（TIMP-2） and transforming growth factor-β1（TGF-β1）which are remodeling related factors in rat injured arteries.Methods：Stenosis models of thoracic aorta after balloon injury was established in rats. Immunohistochemistry was used to evaluate the expression of above factors at different time. Results：All factors were high expressed obviously on the 3rd day, and started to fall to the level of control, respectively. TGF-β1 persisted for 8 d＇ays,MMP-1 for 14 days,MMP-2 for 21 days,while TIMP-2 still kept higher than control till 21st day.Conclusion：All these factors have different time change curve in rat,the first 21 days after injury maybe the optimal period for drug intervention.
出处
《中日友好医院学报》
2007年第2期91-93,F0004,共4页
Journal of China-Japan Friendship Hospital
基金
中日友好医院院级课题
关键词
大鼠
动脉内膜损伤
基质金属蛋白酶
球囊成形术
血管再狭窄
rats
arterial intimal injury
matrix mettalloproteinases
vascular restenosis
balloon angioplasty
