摘要
目的取钉螺血淋巴细胞制备染色体,便于研究钉螺分类、遗传特性及筛选抗性株钉螺。方法将秋水仙素作用后的钉螺血淋巴细胞进行低渗、固定、滴片和染色,显微镜下阅片。结果江苏省扬中江滩地区钉螺的染色体数2n=34,核型公式为14m+8Sm+8St+2t+性染色体。结论用钉螺血淋巴细胞制备染色体,方法简便,图像清晰,可读性好,便于进行核型分析。
In order to prepare chromosome of homeocytr of Oncomelania hupensis for the classification,hereditary character and to screen resistant strain, Homeocyte of Oncomelania hupensis exposed to Colchicine was performed with following procedures： hypotesion, fixation,dropping slide, staining and finally reading under microscope. The chromosome number of Oncomelanla hupensis in Yangzhong of Jangsu is 34, and it＇s karyotype formula is 14m＋8Sm＋8St＋2t ＋ sex chromosome. It indicates that the method can be used in karyotype analysis of Oncomelania hupensis.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2007年第4期384-385,共2页
Chinese Journal of Zoonoses
关键词
钉螺
血淋巴细胞
染色体
核型
Oncomelania hupensis, homeocyte, chromosome, karyotype
