摘要
枯草芽胞杆菌WSH-062在摇瓶培养中能产生胞外的PVA降解酶。通过单因素及正交实验对其进行了发酵条件的优化。研究结果表明:该菌产生的PVA降解酶为诱导型酶;2 g/L的复合氮源(NaNO3和酵母粉的配比为1∶1)就能满足细胞生长和产酶的营养需要。正交实验分析得到最优的发酵培养基为:PVA 30 g/L,酵母粉12.2 g/L,NaNO3 10.1 g/L,MgSO4.7H2O 0.35 g/L,KH2PO4 3 g/L,pH值7.2。优化之后的PVA降解酶酶活达到5.00 U/mL,比优化前提高了4.75倍,并且重复性较好。
Bacillus subtilus WSH-062 could produce extracellular poly （vinyl alcohol） （PVA）-degrading enzyme （PVAase） in the flask culture. Optimization of culture conditions was carried out through mona-factor and orthogonal experiments. The results showed that the enzyme produced by this strain was an induced enzyme. The mixed nitrogen sources （the ratio of NaNO3 to yeast extract was 1：1） with concentration of 2 g/L were favorable for cell growth and enzyme synthesis. The results of orthogonal experiment showed that the optimal fermentation conditions were： PVA 30 g/L, yeast extract 8.2 g/L, NaNO3 10.1 g/L, MgSO4·7H2O 0.35 g/L and KH2PO4 3 g/L at pH 7.2. Under this condition, the maximal PVAase activity was about 5.0 U/mL, which was increased by 4.75-fold compared with the value before optimization.
出处
《化工进展》
EI
CAS
CSCD
北大核心
2007年第4期567-571,共5页
Chemical Industry and Engineering Progress
基金
教育部新世纪优秀人才支持计划资助
江苏省高技术研究项目(No.BG2005016)
关键词
聚乙烯醇
降解酶
发酵条件
优化
poly （vinyl alcohol）
degrading enzyme
fermentation
optimization
