摘要
从工厂废水中分离到一株高效降解聚乙烯醇(PVA)的细菌D_8株,四天能将培养基中0.5%的PVA(500,1700)完全降解,经鉴定为假单胞菌属类产碱假单胞菌(Pseudomonos pseudoalcaligenes)。对菌株的发酵条件进行了研究,结果表明,最适培养基成份为(%):PVA1.5,(NH_4)_2SO_4 0.1,K_2HPO_4 0.24,KH_2PO_4 0.04,MgSO_4·7H_2O 0.035,NaCl 0.01,FeSO_4 0.001,酵母膏0.15;起始pH 7.5;通气量在250ml三角瓶装30ml培养基为最适,于30℃,160r/min的旋转摇床振荡培养72h产酶活力最高。
A bacterium D_8 strain which high effiently degrading PVA was isolated from wester water of factory. The strain possesses the abilites of completely degrading 0.5 per cent of PVA (500, 1700) included in the culture medium for four days. It was identified Pseudomonos pseudoalcaligenes.
Fermentation conditions of the strain have been investigated. The suitable medium consisted of PVA 1.5%, (NH_4)_2SO_4 0.1%, K_2HPO_4 0.24%, KH_2PO_4 0.04%, MgSO_4·7H_2O 0.035%, NaCl 0.01%, FeSO_4 0.001%, yeast extract 0.15%, pH7.5. The optimal condition for enzyme production are as follows: 250ml shake filled with 30ml medium, 30℃, 160n/min incubation period 72h. Under such conditions enzyme activity is highest.
出处
《微生物学报》
CAS
CSCD
北大核心
1989年第5期343-347,共5页
Acta Microbiologica Sinica
关键词
聚乙烯醇
降解酶
产生菌
分离
Polyvinyl Alcohol
Polyvinyl alcohol-degrading enzyme
Secondary alcohol oxidase
Pseudomonas pseudoalcaligenes
