摘要
目的检测类风湿关节炎(RA)患者的外周血和关节滑液中T细胞表达CD25和CD69水平,并探讨它们在关节炎症发生过程中的免疫病理作用。方法于2005年4月-2006年9月间,采集了30例RA患者和12例健康人外周血和膝关节滑液标本,利用流式细胞仪检测T细胞CD25、CD69表达,分析它们在疾病发展过程中的作用。结果RA患者外周血T细胞CD25、CD69表达稍有升高,但与健康者比较无统计学差异(P>0.05);而RA患者的关节滑液中T细胞表达CD25、CD69水平明显高于健康对照者(P<0.05)。12例RA患者接受免疫抑制剂治疗,2个月后分析发现患者的T细胞表达CD25、CD69水平显著下降(P<0.05)。结论RA患者关节滑液中T细胞表达CD25、CD69水平明显升高,提示在关节炎症发生时,T细胞激活、增殖,并直接参与了关节滑膜组织的免疫应答。
Objective To analyze the expression CD25 and CD69 on T cells in peripheral blood and synovial fluid （SF） from patients with rheumatoid arthritis （RA）, and study their role in the pathogenesis of synovial inflammation. Methods From April 2005 to September 2006, 30 patients with RA and 12 healthy controls were enrolled, and peripheral sera and SF were taken subsequently. Expression of CD25 and CD69 on T cells were analyzed on a flow cytometry. Results Expression CD25 and CD69 was slightly increased in peripheral blood of RA patients, but not significantly different than that in controls （ P 〉0. 05）. However, CD25 and CD69 expression was found to be significantly increased in synovial T cells from RA patients compared with those in healthy controls （ P 〈 0.05）. 12 RA patients were treated with immune suppressants, and CD25 and CD69 expression in T cells was also detected 2 months after effective treatment, showing that CD25 and CD69 were markedly decreased （ P 〈 0. 05 ）. Conclusion CD25 and CD69 are significantly increased in synovial T cells from RA patients. These activated T cells may directly participate in immune response in synovial tissue.
出处
《医药论坛杂志》
2007年第3期9-10,13,共3页
Journal of Medical Forum
