摘要
[目的]观察益骨汤含药血清对大鼠成骨细胞增殖和成骨细胞ALP的影响。[方法]取12周龄Wister大鼠40只,雌雄兼用,随机分为A、B、C、D、E五组,每组8只,分为益骨汤高、中、低剂量组(A、B、C组),a-D3对照组(D组)及NS空白对照组(E组),制备成益骨汤含药血清备用。成骨细胞建立采用新生SD乳鼠5只(24h内颅盖骨)进行体外细胞传代培养。观察益骨汤含药血清对体外培养的新生SD大鼠成骨细胞增殖、分化情况。[结果]A、B、C、D组比较无显著性差异,与E组比较细胞增殖分化与ALP影响具有显著性差异(P<0.05)。[结论]益骨汤含药血清具有促进早期成骨细胞的增殖与分化。
[Objective]To investigate the effects of YIGUTANG contaning serum on osteoblasts proliferation which was from the skull of newborn SD rat in vitro. [Methods] The osteoblast from newborn SD rats＇skull adopted, take the method of collagenasepancreatic enzyme digestion,then respectively culture these osteoblasts with different concentration of the YIGUTANG drug containing serum fluid together. [Results]YIGUTANG drug containing serum could stimulate the proliferation of osteoblast, and the high,the middle and the low concentrations groups contrasted with the control group, all could promote the proliferation of cell. The drug containing serum groups had insignificant difference from the blank control group（P〈0.05）. [Conclusion]YIGUTANG can promote the proliferation and the differentiation of osteoblast in vitro in early stage and promote bone formation.
出处
《浙江中医药大学学报》
CAS
2007年第2期158-159,共2页
Journal of Zhejiang Chinese Medical University
基金
浙江省中医药科研基金资助项目(No2005C008)~~
