摘要
[目的]观察仙鹿健骨汤含药血清对大鼠成骨细胞增殖和成骨细胞ALP的影响。[方法]取12周龄Wister大鼠40只,随机分为A、B、C、D、E、F六组,每组8只,分为仙鹿健骨汤低、中、高剂量组(A、B、C组),阳性对照组(D组)、模型组(E组)及空白对照组(F组),制备成仙鹿健骨汤含药血清备用。成骨细胞建立采用新生SD乳鼠5只(24h内颅盖骨)进行体外细胞传代培养。观察仙鹿健骨汤含药血清对体外培养的新生SD大鼠成骨细胞增殖、分化情况。[结果]B、C、D、F组间比较无显著性差异,与E组比较细胞增殖分化与ALP影响具有显著性差异(P<0.05或P<0.01)。[结论]仙鹿健骨汤含药血清能促进大鼠早期成骨细胞的增殖与分化。
[Objective] To observe the effect of Xianlu Jiangu Decoction serum on osteobiasts proliferation and ALP. [Method] Take 40 Wister rats of 12w, randomly divide them into six groups： A,B,C,D,E and F, 8 in each, i.e. low, middle and high-dosage groups（A,B,C） of Xianln Jiangu Decoction, positive control group（D）, model group（E） and blank control group（F）, make the decoction serum for standby. For osteoblasts, take 5 newborn rats for cell passage culture in vitro. Observe the effect of serum on SD rats＇ osteoblasts proliferation and differentiation. [Result] There＇s no marked difference among B,C,D and F, but it has obvious difference compared with E group on proliferation, differentiation and ALP（P〈 0.05 or P〈0.01）. [Conclusion] Xianlu Jiangu Decoction serum can promote rats early osteoblasts proliferation and differentiation.
出处
《浙江中医药大学学报》
CAS
2009年第1期36-37,共2页
Journal of Zhejiang Chinese Medical University
基金
温州市科技局科研基金项目(No:Y20070152)~~
关键词
骨质疏松症
仙鹿健骨汤
成骨细胞
碱性磷酸酶
大鼠
osteoporosis
Xianlu Jiangu decoltion
osteoblasts
ALP
experimental Study
rats
