摘要
目的研究过量碘摄入对小鼠仔鼠脑甲状腺激素受体基因表达的影响及硒的干预作用。方法将60只BALB/c小鼠随机分为4组:正常对照组(饮用自来水,NC)、碘过量组(饮水3000μg/LI,EI+)、单独补硒组(饮水200μg/LSe,Se+)和碘过量加硒组(饮水3000μg/LI+200μg/LSe,EI+Se+)。以纯系鼠饲料饲养。4个月后,雌雄交配。测定14和28日龄仔鼠血清总T4(T4)和总T3(T3)水平,用实时荧光定量PCR测定0、14和28日龄仔鼠大脑组织TRα1和TRβ1mRNA的表达水平。结果14日龄仔鼠血清T4水平碘过量组显著低于对照组和碘过量加硒组(P<0.05)。0和14日龄仔鼠脑组织TRα1和TRβ1mRNA水平碘过量组显著高于对照组、单独补硒组和碘过量补硒组(P<0.05)。碘过量和补硒对28日龄仔鼠血清T4水平和脑组织TRα1和TRβ1表达水平无明显影响。结论碘过量上调子代脑TRα1和TRβ1的表达,补硒具有缓解作用。
Objective To study effects of iodine excess on the levels of serum T4 and Ts and TRα1 and TRβ1 expression in the cerebrum of filial mice and the supplement of selenium. Methods Sixty BALB/c mice were divided randomly into four groups： control group （tap water, NC）, iodine excess group （3000μg/L I,EI ＋ ） ,selenium supplement group （200μg/L Se, Se ＋ ） and iodine excess plus selenium（3000μg/L ＋ I 200μg/L Se, EI ＋ Se ＋ ） group. The mice were mated at the end of the fourth month. Serum T4 and T3 were determined on postnatal day 14 and 28. The expression levels of TRα1 and TRβ1 mRNA in filial mice cerebrum were detected by fluor scent real time PCR. Results Serum T4 level in EI ＋ was lower significantly than that in NC and EI ＋ Se ＋ on postnatal day 14. mRNA levels of TRα1 and TRβ1 in EI ＋ were higher than those in other groups on postnatal day 0 and 28. No significant difference in serum T4 level and TRα1 and TRβ1 mRNA expression level between four group on postnatal day 28. Conclusion The expression of TRα1 and TRβ1 infilial mice cerebrum could be up-regulated by iodine excess intake and could be alleviated by selenium supplement.
出处
《卫生研究》
CAS
CSCD
北大核心
2007年第2期175-177,共3页
Journal of Hygiene Research
基金
国家自然科学基金重点项目资助No.30230330)
湖北省教育厅基金资助No.2002A03007
关键词
碘过量
硒
甲状腺激素受体
仔鼠
大脑
iodine excess, selenium supplement, thyroid hormone receptor, filial mice, cerebrum
