摘要
以红金银花顶芽和叶片为实验材料，采用L16（4^4×2^3）混合水平的正交试验设计，对红金银花诱导愈伤组织进行了研究，结果表明红金银花外植体类型、培养基类型、6-BA浓度、NAA浓度以及蔗糖浓度和培养温度对愈伤组织的产生均有影响；顶芽诱导率高于叶片诱导率，MS＋6-BA0．5mg／L＋NAA0．2mg／L为最佳培养基；最适蔗糖浓度为30．0g／L，最适温度为23℃～25℃。
Abstract： Terminal buds and leaves of Lonicera japonica var. chinensis were studied for inducing callus, through the orthogonal design L16 （4^4×2^3） . The five facts, the explant of Lonicera japonica vat. chinensis, medium, temperature, concentration of 6 - BA, NAA and sucrose were studied. The results showed that all of the five factors had significant effects on callus induction. The inducing rate of terminal bud was higher than the inducing rate of leaves. The best medium for callus induction was MS ＋ 6 - BA0.5mg/L ＋ NAA0.2mg/L. The best concentration of sucrose was 30.0g/L and the best temperature was 23 - 25℃.
出处
《山东林业科技》
2007年第1期9-11,共3页
Journal of Shandong Forestry Science and Technology
关键词
红金银花
组织培养
愈伤组织
正交试验
Lonicera japonica vat. ehinensis
tissue culture
callus
orthogonal design
