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内毒素体外诱生人单核细胞分泌PGF1α/TXB_2的研究 被引量:1

Research on the Secretion of PGF_1α/TXB_2 in Human Monocytes Induced by Endotoxin in Vitro
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摘要 分离健康人外周血单核细胞,行短期体外培养后,以大肠杆菌内毒素刺激单核细胞,放免法测定细胞培养上清液中前列腺素F1α血栓素B2含量。结果表明内毒素体外能诱生人外周血单核细胞合成PGF1α/TXB2,内毒素刺激量为10μg/ml时单核细胞合成PGF1α/TXB2最旺盛,刺激2小时左右合成速率达到高峰,随后下降。 Supernantant PGF1α & TXB2 Lvels of endotoxin-induced adult human monocytes were measured by the method of radioimmunoassay.It was observed that endotoxin could improve the monocytes PGF1α and TXB2 secretion in vitro.The data showed that the concentration of PGF1α and TXB2 secreted by monocytes reached a peak level when the final concentration of endotoxin was 10μg/ml and the stimulated time lasted to 2 hours.
机构地区 天津市南开医院
出处 《中国中西医结合外科杂志》 CAS 1996年第3期173-175,共3页 Chinese Journal of Surgery of Integrated Traditional and Western Medicine
关键词 单核细胞 内毒素 前列腺素F1Α 血栓素B2 monocyte,endotoxin, Prostagland F_(1α), thromboxin B_2
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